Open Access Research

Decline of nucleotide excision repair capacity in aging Caenorhabditis elegans

Joel N Meyer1, Windy A Boyd2, Gregory A Azzam1, Astrid C Haugen1, Jonathan H Freedman2 and Bennett Van Houten1*

Author Affiliations

1 Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Alexander Drive, Research Triangle Park, NC 27709, USA

2 Laboratory of Molecular Toxicology, National Institute of Environmental Health Sciences, Alexander Drive, Research Triangle Park, NC 27709, USA

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Genome Biology 2007, 8:R70  doi:10.1186/gb-2007-8-5-r70

Published: 1 May 2007

Additional files

Additional data file 1:

Provided are the specifics of the C. elegans amplicons used for QPCR (genomic target amplified, size of QPCR target, typical cycle number for quantitative analysis, forward and reverse primers, and annealing temperature).

Format: XLS Size: 21KB Download file

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Open Data

Additional data file 2:

Shown are photographs of young (1-day-old) and aging (6-day-old) glp-1 adults raised at 25°C, at 10× and 20× magnification. Hollow arrows point to tails without blisters, and solid arrows point to blisters.

Format: TIFF Size: 1.1MB Download file

Open Data

Additional data file 3:

Provided are lists of altered Gene Ontologies (cellular component, molecular function, and biologic process) identified by GOMiner, when comparing young (1-day) with aging (6-day) glp-1 adult C. elegans raised at 25°C.

Format: XLS Size: 67KB Download file

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Open Data

Additional data file 4:

Part A lists the 20 top-scoring active modules representing altered gene neighborhoods identified by Cytoscape, when comparing young (1-day) with aging (6-day) glp-1 adult C. elegans raised at 25°C. Part B lists the results of BiNGO analysis of the 20 top-scoring active modules representing altered gene neighborhoods identified by Cytoscape, when comparing young (1-day) with aging (6-day) glp-1 adult C. elegans raised at 25°C.

Format: XLS Size: 517KB Download file

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Open Data

Additional data file 5:

Presented are the results of BiNGO analysis of the entire dataset comparing young (1-day) with aging (6-day) glp-1 adult C. elegans raised at 25°C.

Format: XLS Size: 321KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data