Validation of microarray data by qRT-PCR. The mean fold changes of mRNA expression in postnatal day (P)16 Nfia-/- relative to Nfia+/+ mice are given on the y-axis. Numbers of independent samples: n = 3 for microarray analysis of Nfia-/- and Nfia+/+ each; n = 8 for quantitative real-time polymerase chain reaction (qRT-PCR) analysis of Nfia-/-; and n = 6 for qRT-PCR analysis of Nfia+/+. Samples used for qRT-PCR include those used in the microarray investigation. Adjacent bars show the microarray and qRT-PCR results for the respective gene. For all 15 genes investigated, the microarray and the qRT-PCR results follow the same direction (either upregulation or downregulation). All differences observed by qRT-PCR are statistically significant (P < 0.001 for Gabra6, GFAP, Mal, Dio2, Mobp, Sox11, and Nnat; P < 0.01 for MAG, Mog, Sox2, Sox4, Dcx, and TN-C; and P < 0.05 for Car2 and Plp1). Car2, carbonic anhydrase 2; Dcx, doublecortin; Dio2, iodothyronine deiodinase II; Gabra6, α6 subunit of γ-aminobutyric acid type A receptor; GFAP, glial fibrillary acidic protein; MAG, myelin-associated glycoprotein; Mal, myelin and lymphocyte protein; Mobp, myelin oligodendrocyte basic protein; Mog, myelin oligodendrocyte glycoprotein; Nnat, neuronatin; Plp1, proteolipid protein (myelin) 1; Sox2, SRY-box containing gene 2; Sox4, SRY-box containing gene 4; Sox11, SRY-box containing gene 11; TN-C, tenascin-C.
Wong et al. Genome Biology 2007 8:R72 doi:10.1186/gb-2007-8-5-r72