Figure 2.

EvoPrint analysis of vertebrate Delta-like 1 enhancers. An EvoPrint of the vertebrate Dll1 cis-regulatory region generated from the following genomes: mouse (reference sequence), human, rhesus monkey, cow, rat, opossum and Xenopus tropicalis. Shown is the first codon (ATG) and 4,265 bp of upstream 5' flanking sequence of the mouse Dll1 gene containing, in 5' → 3' order, respectively, the Homology-I neural enhancer region (304 bp), the msd mesodermal enhancer (a 1,495 bp FokI restriction fragment), the Homology-II neural enhancer (207 bp fragment) and the msd-II mesodermal enhancer (1,615 bp HindIII restriction fragment) as described [35]. Multi-species conserved sequences within the murine DNA, shared by all orthologous DNAs that were used to generate the EvoPrint, are identified with uppercase black-colored letters and less or non-conserved DNA are denoted by lowercase gray-colored letters. Note that the chimpanzee, dog and chicken genomes were excluded from the analysis due either to sequence breaks and/or sequencing ambiguities as detected by EvoDifference profiles.