Table 3 |
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|
Differential expression data based on array experiments and Q-PCR of genes with unknown or ubiquitous expression pattern |
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|
Other genes |
Expression |
Regulation |
P Value |
Q-PCR ratio |
|
|
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|
Arginine kinase 536REAsu4_15B20 |
? |
Li 2.20 Zn na |
7.29 × e-07 na |
1.95 ± 0.15 2.07 ± 0.47 |
|
ß-catenin 538REA_2O22 RUDIREA_22E13 |
Ubiquitous |
Li 0.39 Zn na |
4.77 × e-03 na |
0.85 ± 0.11 0.79 ± 0.16 |
|
SpZ12 |
? |
Li 1.44 Zn 2.29 |
2.55 × e-01 2.05 × e-02 |
2.03 ± 0.37 2.66 ± 0.40 |
|
Hmx, NkX5.1 537REA_6C02 |
? |
Li 0.20 Zn 1.86 |
1.18 × e-14 5.87 × e-03 |
0.05 ± 0.03 1.20 ± 0.32 |
|
Tcf/Lef 536REAsu4_11P24 |
? |
Li 1.37 Zn na |
7.88 × e-02 na |
0.73 ± 0.13a 1.42 ± 0.29 |
|
WntA RUDIREA_33L4 |
? |
Li 1.07 Zn nd |
8.13 × e-01 nd |
0.39 ± 0.13a 0.24 ± 0.12 |
|
Wnt1 |
? |
Li na Zn na |
na na |
ne ne |
|
Wnt4 536REAsu4_6C19 |
? |
Li 1.09 Zn NA |
6.69 × e-01 NA |
1.66 ± 0.36 1.73 ± 0.21 |
|
Wnt6 |
? |
Li na Zn na |
na na |
1.55 ± 0.19 0.24 ± 0.01 |
|
Wnt7 |
? |
Li na Zn na |
na na |
ne 0.03 ± 0.02 |
|
Wnt9 |
? |
Li na Zn na |
na na |
ne 0.29 ± 0.03 |
|
Wnt10 |
? |
Li na Zn na |
na na |
1.43 ± 0.29 1.30 ± 0.14 |
|
Wnt16 |
? |
Li na Zn na |
na na |
2.79 ± 0.81a 0.84 ± 0.12 |
|
|
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|
The first column gives the gene name and the clone ID, both of which can be used to query the described database [22] for additional data. In the second column the localization of expression in the embryo is given. The third column (Regulation) gives the differential expression ratios (expression in treatment/expression control) based on the array experiment for lithium (Li) and zinc (Zn) treated embryos (values above 1 indicate upregulation and values below 1 indicate downregulation). The column 'P value' indicates the statistical probability that the regulation could happen by chance (see Materials and methods for detail). The column Q-PCR (quantitative real-time polymerase chain reaction) gives the differential expression ratios (expression in treatment/expression control) and the error, as determined by Q-PCR. (Values expressed in copies of mRNA molecules/embryo are provided via the expressed sequence tag database [75]; see Materials and methods for details on Q-PCR). aDifferential expression based on array and Q-PCR data do not correlate. na, not analyzed; nd, no statistically relevant differential expression; ne, not expressed; ?, expression pattern unknown. |
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|
Poustka et al. Genome Biology 2007 8:R85 doi:10.1186/gb-2007-8-5-r85 |
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