Table 3

Differential expression data based on array experiments and Q-PCR of genes with unknown or ubiquitous expression pattern

Other genes

Expression

Regulation

P Value

Q-PCR ratio


Arginine kinase

536REAsu4_15B20

?

Li 2.20

Zn na

7.29 × e-07

na

1.95 ± 0.15

2.07 ± 0.47

ß-catenin

538REA_2O22 RUDIREA_22E13

Ubiquitous

Li 0.39

Zn na

4.77 × e-03

na

0.85 ± 0.11

0.79 ± 0.16

SpZ12

?

Li 1.44

Zn 2.29

2.55 × e-01

2.05 × e-02

2.03 ± 0.37

2.66 ± 0.40

Hmx, NkX5.1

537REA_6C02

?

Li 0.20

Zn 1.86

1.18 × e-14

5.87 × e-03

0.05 ± 0.03

1.20 ± 0.32

Tcf/Lef

536REAsu4_11P24

?

Li 1.37

Zn na

7.88 × e-02

na

0.73 ± 0.13a

1.42 ± 0.29

WntA

RUDIREA_33L4

?

Li 1.07

Zn nd

8.13 × e-01

nd

0.39 ± 0.13a

0.24 ± 0.12

Wnt1

?

Li na

Zn na

na

na

ne

ne

Wnt4

536REAsu4_6C19

?

Li 1.09

Zn NA

6.69 × e-01

NA

1.66 ± 0.36

1.73 ± 0.21

Wnt6

?

Li na

Zn na

na

na

1.55 ± 0.19

0.24 ± 0.01

Wnt7

?

Li na

Zn na

na

na

ne

0.03 ± 0.02

Wnt9

?

Li na

Zn na

na

na

ne

0.29 ± 0.03

Wnt10

?

Li na

Zn na

na

na

1.43 ± 0.29

1.30 ± 0.14

Wnt16

?

Li na

Zn na

na

na

2.79 ± 0.81a

0.84 ± 0.12


The first column gives the gene name and the clone ID, both of which can be used to query the described database [22] for additional data. In the second column the localization of expression in the embryo is given. The third column (Regulation) gives the differential expression ratios (expression in treatment/expression control) based on the array experiment for lithium (Li) and zinc (Zn) treated embryos (values above 1 indicate upregulation and values below 1 indicate downregulation). The column 'P value' indicates the statistical probability that the regulation could happen by chance (see Materials and methods for detail). The column Q-PCR (quantitative real-time polymerase chain reaction) gives the differential expression ratios (expression in treatment/expression control) and the error, as determined by Q-PCR. (Values expressed in copies of mRNA molecules/embryo are provided via the expressed sequence tag database [75]; see Materials and methods for details on Q-PCR). aDifferential expression based on array and Q-PCR data do not correlate. na, not analyzed; nd, no statistically relevant differential expression; ne, not expressed; ?, expression pattern unknown.

Poustka et al. Genome Biology 2007 8:R85   doi:10.1186/gb-2007-8-5-r85

Open Data