Method
Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes
-
* Corresponding author: Hyunwoo Lee hlee31@uic.edu
1 Center for Pharmaceutical Biotechnology, University of Illinois, Chicago, Illinois 60607, USA
2 Current address: Department of Biochemistry, New York University School of Medicine, New York, New York 10016, USA
3 Deceased (20 April 2006)
Genome Biology 2007, 8:R87 doi:10.1186/gb-2007-8-5-r87
Published: 22 May 2007Additional files
Additional data file 1:
Presented is a table containing microarray raw data from the aromatic amino acid selection performed with the defined library and log ratios of probes calculated with normalized signal intensities.
Format: ZIP Size: 4MB Download file
Additional data file 2:
Presented is a table containing microarray raw data from the aromatic amino acid selection performed with the random library and log ratios of probes calculated with normalized signal intensities.
Format: ZIP Size: 4.6MB Download file
Additional data file 3:
Presented is a table containing microarray raw data from the chloramphenicol selection performed with the defined library and log ratios of probes calculated with normalized signal intensities.
Format: ZIP Size: 4MB Download file
Additional data file 4:
Presented is a figure illustrating the biased representation of transposon mutants in the random transposon insertion library used in this study.
Format: PDF Size: 8.9MB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional data file 5:
Presented is a figure showing the chromosomal location of genes identified in the aromatic amino acid selection with the defined and random library.
Format: PDF Size: 233KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional data file 6:
Presented is a figure showing optimized parameters of microarray target preparation in MGK.
Format: PDF Size: 4.3MB Download file
This file can be viewed with: Adobe Acrobat Reader