Genome Biology
Volume 8
Issue 5

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Gomez MJ
Shatalin KY
Lee H
Neyfakh AA

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Shatalin KY
Lee H
Neyfakh AA
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Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes

Lisa K Smith¹ , Maria J Gomez¹ , Konstantin Y Shatalin² , Hyunwoo Lee¹ and Alexander A Neyfakh¹^,3

¹Center for Pharmaceutical Biotechnology, University of Illinois, Chicago, Illinois 60607, USA

²Current address: Department of Biochemistry, New York University School of Medicine, New York, New York 10016, USA

³Deceased (20 April 2006)

author email corresponding author email

Genome Biology 2007, 8:R87doi:10.1186/gb-2007-8-5-r87


Published:	22 May 2007

Subject areas: Microbiology and parasitology, Genome studies, Genetics

Additional files

Additional data file 1:

Presented is a table containing microarray raw data from the aromatic amino acid selection performed with the defined library and log ratios of probes calculated with normalized signal intensities.

Format: ZIP Size: 4.1MB Download file

Additional data file 2:

Presented is a table containing microarray raw data from the aromatic amino acid selection performed with the random library and log ratios of probes calculated with normalized signal intensities.

Format: ZIP Size: 4.8MB Download file

Additional data file 3:

Presented is a table containing microarray raw data from the chloramphenicol selection performed with the defined library and log ratios of probes calculated with normalized signal intensities.

Format: ZIP Size: 4.1MB Download file

Additional data file 4:

Presented is a figure illustrating the biased representation of transposon mutants in the random transposon insertion library used in this study.

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Additional data file 5:

Presented is a figure showing the chromosomal location of genes identified in the aromatic amino acid selection with the defined and random library.

Format: PDF Size: 233KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional data file 6:

Presented is a figure showing optimized parameters of microarray target preparation in MGK.

Format: PDF Size: 4.4MB Download file

This file can be viewed with: Adobe Acrobat Reader