Table 1 |
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|
Primers used in this study |
|
| Primer name |
Sequence |
|
|
|
| For defined library |
|
|
|
|
| Up-BIO |
5'-biotin-GAACTTCGAACTGCAGGTCGAC-3' |
| Dn-BIO |
5'-biotin-GTATAGGAACTTCGAAGCAGCTC-3' |
| Up-Cy3 |
5'-GGTCGACGGACCCCCG-3' |
| Up-Cy5 |
5'-GGTCAACGGATCCCCG-3' |
| Dn-Cy3 |
5'-AAGCAGTTCCAGCCTACA-3' |
| Dn-Cy5 |
5'-AAGCAGCTCCAGTCTACA-3' |
|
|
|
| For random library |
|
|
|
|
| Tn10OE-BIO |
5'-biotin-CAAGATGTGTATCCACCTTAACTTAA-3' |
| Tn10OE OUT-Cy3 |
5'-ACCAATATCATTAGGGGAT-3' |
| Tn10OE OUT-Cy5 |
5'-ACCAAAATCATAAGGGGAT-3' |
|
|
|
| Common for both libraries |
|
|
|
|
| TATV-3 or Oligo(dT9 AT15V) |
5'-T9 AT15V-3' |
| TATV-5 or Oligo(dT15 AT9V) |
5'-T15 AT9V-3' |
|
|
|
|
Mismatched nucleotides are in bold. 'V' represents A, C, or G. |
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|
Smith et al. Genome Biology 2007 8:R87 doi:10.1186/gb-2007-8-5-r87 |
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