Table 2 |
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|
List of genes identified by MGK as important for growth in the absence of aromatic amino acids |
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| Mutant library used |
Genes |
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|
|
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| Defined |
trpAa (28.1) |
pheAa (26.1) |
epda (23.8) |
trpEa (22.8) |
aroEa (20.1) |
| tyrAa (17.5) |
aroCa (14.8) |
trpBa (13.9) |
aroAa (13.3) |
aroBa (11.9) |
|
| tyrBa (12.2) |
trpDa (7.4) |
ygcL (6.6) |
rcsF (6.3) |
yddK (5.7) |
|
| ygaF (5.4) |
ynaJ (5.3) |
pdxAa (5.2) |
wzc (5.0) |
surA (4.9) |
|
| rpea (4.8) |
ygdDa (4.7) |
aroF (4.1) |
yadN (4.0) |
uvrY (4.0) |
|
| yeeA (4.0) |
ydhH (3.9) |
yfhD (3.9) |
yadK (3.8) |
ompX (3.6) |
|
| aroDa (3.5) |
yfhM (3.5) |
yedV (3.4) |
ybeL (3.4) |
trpCa (3.3) |
|
| wcaA (3.3) |
ydhX (3.2) |
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|
|
|||||
| Random |
tyrBa (15.9) |
epda (13.3) |
trpAa (10.8) |
aroEa (10.4) |
tyrAa (7.8) |
| trpBa (3.9) |
hepA (3.5) |
tktAa (3.2) |
|||
|
|
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|
Values in parentheses are intensity ratios (normalized reference intensity value/normalized selection intensity value), and are the average of two independent, inversely labeled experiments. All mutants were individually tested. aMutants that exhibited a growth defect in the absence of aromatic amino acids. MGK, Monitoring of Gene Knockouts. |
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|
Smith et al. Genome Biology 2007 8:R87 doi:10.1186/gb-2007-8-5-r87 |
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