Table 3 |
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List of genes identified by MGK as important for survival upon chloramphenicol treatment |
|
| Phenotype verification of mutants |
Genes |
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| Not tested |
pstS (10.5), tolA/B (7.2), ptsI (4.9), acrB (4.2), tolA/R (4.2), sufB (3.4) |
| Individually tested mutants |
hns (12.7), dgkA (11.5), rnhA (10.2), apaH (10.0), rluD (8.6), ahpC (8.3), ompA (8.1), pstC (7.7), rfaE (7.7), arcA (6.8), yjjY (6.7), oxyR (6.2), gor (5.5), rfaF (5.0), sufD (5.0), lpp (4.9), prc (4.7), ybeX (4.6), fpr (4.6), acrA (4.5), tolQ (4.5), arcB (4.0), phoP (3.9), clpA (3.7), ydhD (3.6), mdh (3.5), yqiC (3.5), miaA (3.4), ptsH (3.4) |
| Individually tested mutants exhibiting a fivefold or greater killing with 18-hour
exposure to chloramphenicol |
dgkA (11.5), apaH (10.0), ahpC (8.3), ompA (8.1), arcA (6.8), yjjY (6.7), lpp (4.9), prc (4.7), ybeX (4.6), tolQ (4.5), arcB (4.0), clpA (3.7), mdh (3.5) |
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Values in parentheses are intensity ratios (normalized reference intensity value/normalized selection intensity value), and are the average of two independent, inversely labeled experiments. In the case of tolA/B and tolA/R, the origin of signal intensity could not be distinguished between two neighboring genes. MGK, Monitoring of Gene Knockouts. |
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Smith et al. Genome Biology 2007 8:R87 doi:10.1186/gb-2007-8-5-r87 |
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