Table 2

Summary of manual annotations
BAC number
 Transcript number
 Aedes transcript
 Supercontig
 Contig
 Sequencing of cDNA
 Differences in annotation between manual annotation (MA) and gene build
 Replicated transcript in Aedes assembly














Aedes transcript
 Supercontig
 Contig
 Notes
1
 1
 AAEL013103
 1.789
 24718
 -
 -
 AAEL014498
 1.1137
 29191
 -

2
 AAEL013088
 1.789
 24718
 -
 -
 AAEL014499
 1.1137
 29191
 -

3
 AAEL013092
 1.789
 24718
 -
 -
 AAEL014500
 1.1137
 29189
 -

4
 AAEL013582
 1.875
 25903
 Identical
 Longer 5' in MA
 AAEL015005
 1.1393
 31331
 5' end of transcript matched MA







AAEL013099
 1.789
 24718
 3' end of transcript matched MA

5*
 AAEL015006
 1.1393
 31331
 Identical
 -
 AAEL013097
 1.789
 24718
 Identical to AAEL013097







AAEL013583
 1.875
 25903
 Identical to AAEL013583

6
 AAEL013098
 1.789
 24719
 -
 Only 3' coding region lines up
 AAEL013098
 1.789
 24719
 Only 3' coding region lines up
3
 7*
 AAEL009524
 1.403
 16729
 -
 -
 AAEL000392
 1.7
 624
 -
4
 8
 AAEL008104
 1.301
 13837
 -
 -
 -
 -
 -
 -

9
 AAEL008110
 1.301
 13837
 Identical
 Different intron/exon structure
 -
 -
 -
 -

10
 AAEL008114
 1.301
 13837
 Identical
 3' longer in MA
 -
 -
 -
 -

11
 AAEL008115
 1.301
 13837
 -
 -
 -
 -
 -
 -

12
 AAEL008103
 1.301
 13837
 Identical
 -
 -
 -
 -
 -

13
 AAEL008100
 1.301
 13837
 -
 -
 -
 -
 -
 -
5
 14*
 AAEL014561
 1.1166
 29449
 Identical
 -
 Transcript missing
 1.216
 10858
 -

15
 AAEL014559
 1.1166
 29447
 Identical
 -
 AAEL006682
 1.216
 10863
 -
6
 16*
 AAEL014711
 1.1232
 30069
 Identical
 Different intron/exon structure
 AAEL014491
 1.1132
 29135
 -

17
 AAEL014712
 1.1232
 30070
 -
 -
 AAEL014495
 1.1132
 29137
 -

18
 AAEL014709
 1.1232
 30070
 Identical
 -
 AAEL014494
 1.1132
 29136
 -
7
 19
 AAEL006423
 1.204
 10417
 -
 -
 -
 -
 -
 -

20
 No transcript
 1.204
 10417
 -
 Similar to AAEL003685
 -
 -
 -
 -

21*
 AAEL006424
 1.204
 10417
 Identical
 -
 -
 -
 -
 -

22
 AAEL006417
 1.204
 10417
 Identical
 -
 -
 -
 -
 -
8
 23*
 AAEL006019
 1.186
 9724
 Identical
 -
 AAEL008297
 1.312
 14138
 -
9
 24*
 AAEL010573
 1.488
 18854
 Identical
 -
 AAEL000068
 1.1
 48
 -

25
 AAEL010594
 1.488
 18854
 Identical
 -
 AAEL000046
 1.1
 48
 -

26
 AAEL010587
 1.488
 18855
 Identical
 -
 AAEL000020
 1.1
 48
 -

27
 AAEL010575
 1.488
 18855
 Identical
 -
 AAEL000076
 1.1
 48
 -

28
 AAEL000054
 1.1
 48
 -
 -
 AAEL010578
 1.488
 18855
 -

29
 AAEL010595
 1.488
 18855
 -
 -
 -
 -
 -
 -
10
 30
 AAEL007897
 1.288
 13401
 Identical
 Extra 3' exon in MA
 -
 -
 -
 -

31
 AAEL007893
 1.288
 13401
 -
 -
 -
 -
 -
 -

32
 AAEL007907
 1.288
 13401
 -
 -
 -
 -
 -
 -
11
 33
 AAEL002503
 1.59
 3864
 -
 -
 -
 -
 -
 -

34
 AAEL002532
 1.59
 3866
 Identical
 3' exon absent in MA
 -
 -
 -
 -

35
 AAEL002534
 1.59
 3863
 -
 -
 -
 -
 -
 -

36
 AAEL002523
 1.59
 3864
 -
 -
 -
 -
 -
 -
12
 37
 AAEL001205
 1.25
 1764
 -
 Longer 5' in MA
 -
 -
 -
 -

38
 AAEL001215
 1.25
 1764
 Identical
 Longer 3' in MA
 -
 -
 -
 -

39
 AAEL001215
 1.25
 1764
 Identical
 -
 -
 -
 -
 -

40
 AAEL001198
 1.25
 1761
 -
 -
 -
 -
 -
 -

41
 AAEL001201
 1.25
 1764
 -
 -
 -
 -
 -
 -

42
 AAEL001210
 1.25
 1764
 -
 -
 -
 -
 -
 -
13
 43
 AAEL008780
 1.348
 15270
 -
 -
 AAEL001693
 1.39
 2713
 -

44
 AAEL008781
 1.348
 15269
 -
 -
 AAEL001703
 1.39
 2712
 -

45
 AAEL008769
 1.348
 15269
 -
 -
 AAEL001701
 1.39
 2711
 -

46
 AAEL008778
 1.348
 15269
 -
 -
 AAEL001681
 1.39
 2711
 -
14
 47
 AAEL005065
 1.140
 7865
 -
 -
 AAEL005223
 1.146
 8136
 -

48
 AAEL005085
 1.140
 7866
 -
 -
 AAEL005220
 1.146
 8136
 -

49
 AEL005237
 1.146
 8136
 -
 -
 AAEL005088
 1.140
 7865
 -

50
 AAEL005229
 1.146
 8136
 -
 -
 AAEL005059
 1.140
 7865
 -

51
 AAEL005218
 1.146
 8136
 -
 -
 No gene build annotation
 1.140
 7865
 -

The 51 manually annotated transcripts (Transcript number) from each BAC clone (BAC number) along with their corresponding transcript (Gene build transcript) from the gene build (AaegL1) and their location (supercontig, contig) are listed along with cDNA amplicons if sequenced. Transcripts that were replicated in the genome are also listed along with their corresponding gene build transcript, location and differences with the manual annotation (MA) if any. Manual annotations marked with an asterisk indicate single-copy cDNA-derived genetic markers used to isolate the BAC.

Lobo et al. Genome Biology 2007 8:R88   doi:10.1186/gb-2007-8-5-r88

Open Data