Table 2 |
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|
Comparison of microarray and real-time PCR performance in detection of pathogen genera (HRV, pneumovirus) |
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| Patient ID |
Array |
WKL |
P value |
PDA genus diagnosis |
PCR diagnosis |
PCR Ct value |
Virus copy no. |
|
|
|||||||
| 111 |
35915 |
ND |
ND |
||||
| 122 |
35887 |
20.87 |
2.47 × 10-29 |
Pneumovirus |
Pneumovirus |
24.8 |
5.0 × 104 |
| 133 |
71180 |
22.33 |
6.93 × 10-62 |
Pneumovirus |
Pneumovirus |
25.1 |
4.0 × 104 |
| 165 |
66691 |
16.95 |
3.49 × 10-4 |
Pneumovirus |
Pneumovirus |
27.9 |
3.9 × 103 |
| 185* |
66696 |
ND |
ND |
||||
| 254 |
70935 |
25.02 |
2.87 × 10-39 |
Pneumovirus |
Pneumovirus |
22 |
5.4 × 105 |
| 261* |
66697 |
ND |
ND |
||||
| 283* |
63781 |
23.99 |
2.28 × 10-25 |
Pneumovirus |
HRV |
28.3 |
6.1 × 104 |
| 14.07 |
4.66 × 10-11 |
HRV |
|||||
| 312* |
66701 |
ND |
Pneumovirus† |
33.7 |
44 |
||
| 321* |
71006 |
ND |
Pneumovirus† |
31.1 |
340 |
||
| 324* |
35259 |
20.61 |
3.55 × 10-94 |
Pneumovirus |
Pneumovirus |
21.4 |
3.0 × 106 |
| 331* |
66698 |
ND |
HRV |
31.7 |
3.6 × 103 |
||
| 337 |
71192 |
21.73 |
3.49 × 10-14 |
Pneumovirus |
Pneumovirus |
26.2 |
1.1 × 105 |
| 8.3 |
1.92 × 10-4 |
HRV |
HRV |
29.1 |
3.1 × 104 |
||
| 355 |
35662 |
18.00 |
2.97 × 10-40 |
Pneumovirus |
Pneumovirus |
20.3 |
6.7 × 106 |
| 368* |
66702 |
ND |
ND |
||||
| 374 |
66695 |
ND |
Pneumovirus |
34.1 |
500 |
||
| 378 |
70933 |
13.82 |
7.77 × 10-17 |
Pneumovirus |
Pneumovirus |
23.9 |
5.4 × 105 |
| 393* |
71189 |
25.41 |
1.15 × 10-18 |
HRV |
HRV |
30.2 |
2.1 × 105 |
| 412 |
35890 |
19.66 |
2.42 × 10-49 |
Pneumovirus |
Pneumovirus |
23.5 |
6.9 × 105 |
| 414 |
71025 |
49.91 |
1.18 × 10-65 |
Pneumovirus† |
Pneumovirus† |
22.3 |
3.9 × 105 |
| HRV |
33 |
2.6 × 103 |
|||||
| 461 |
66699 |
ND |
ND |
||||
| 478 |
71027 |
ND |
Pneumovirus† |
34.8 |
18 |
||
| 483* |
36053 |
12.17 |
1.47 × 10-12 |
Pneumovirus |
Pneumovirus |
24.8 |
2.9 × 105 |
| 554 |
70997 |
78.55 |
4.59 × 10-120 |
HRV |
HRV |
23.5 |
1.5 × 106 |
| 573 |
66700 |
38.09 |
6.26 × 10-22 |
HRV |
HRV |
22.2 |
3.6 × 106 |
| 639* |
71182 |
9.23 |
7.91 × 10-6 |
HRV |
ND |
||
| 699 |
71007 |
ND |
ND |
||||
| 769 |
73067 |
24.62 |
3.70 × 10-52 |
Pneumovirus |
Pneumovirus |
25.7 |
2.5 × 104 |
| 818 |
70927 |
10.40 |
1.63 × 10-8 |
HRV |
HRV |
34.2 |
1.2 × 103 |
| 832 |
73068 |
13.52 |
4.54 × 10-6 |
Pneumovirus |
Pneumovirus |
28.2 |
3.1 × 103 |
| 40.43 |
1.73 × 10-36 |
Pneumovirus† |
Pneumovirus† |
23.8 |
1.2 × 105 |
||
| 841 |
73070 |
22.11 |
6.80 × 10-50 |
Pneumovirus |
Pneumovirus |
20.9 |
4.5 × 106 |
| 35.4 |
8 |
||||||
| HRV |
29.2 |
3.3 × 104 |
|||||
| 853* |
66690 |
ND |
ND |
||||
| 859 |
71188 |
72.17 |
1.42 × 10-128 |
HRV |
HRV |
24.5 |
2.8 × 106 |
| 892* |
68359 |
12.43 |
5.77 × 10-5 |
HRV |
Pneumovirus |
34 |
27 |
| HRV |
32.3 |
4.2 × 103 |
|||||
| 913 |
71028 |
40.67 |
1.60 × 10-50 |
Pneumovirus† |
Pneumovirus† |
19.1 |
4.7 × 106 |
| 924* |
66703 |
12.79 |
2.56 × 10-6 |
Pneumovirus† |
Pneumovirus† |
31.5 |
250 |
| Pneumovirus |
33.7 |
630 |
|||||
|
|
|||||||
|
*Hospitalized patients. †RSV A patient samples. ND, none detected. |
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|
Wong et al. Genome Biology 2007 8:R93 doi:10.1186/gb-2007-8-5-r93 |
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