Figure 2.

Numerical and functional distribution of PHA genes. The cladogram (main) shows the phylogenetic relationship between the 15 genomes used in this study, ignoring branch length. The topology of the tree is based on whole-genome sequence alignment. The true phylogenetic distance with the respective branch lengths drawn to scale are shown in the phylogram detailed in the inset; the phylogram was built using the Kimura 2-parameter model. Numbers within parentheses (main) reflect the number of PHA genes. Pie charts on each branch represent the functional classification of genes based on the color-scale detailed in the key. The non-phage functional class (black and white downward diagonal color pattern) was introduced to classify CDSs without color-coded functional classification in their annotation; those CDSs assigned into the 'non-phage' pseudo-class represent CDSs that belong to any of the 13 functional classes apart from the phage class. Numbers of genes on branches 1, 2 and 3 reflect the intersection of the respective number of genes determined on each branch using one of the three query genomes; the same applies for genes assigned to branch 4_[TS].