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Open Access Method

RAD marker microarrays enable rapid mapping of zebrafish mutations

Michael R Miller12, Tressa S Atwood13, B Frank Eames2, Johann K Eberhart2, Yi-Lin Yan2, John H Postlethwait2 and Eric A Johnson1*

Author Affiliations

1 Institute of Molecular Biology, University of Oregon, 1370 Franklin Blvd., Eugene, Oregon 97403, USA

2 Institute of Neuroscience, University of Oregon, 1370 Franklin Blvd., Eugene, Oregon 97403, USA

3 FloraGenex, Inc., 1370 Franklin Blvd., Eugene, Oregon 97403, USA

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Genome Biology 2007, 8:R105  doi:10.1186/gb-2007-8-6-r105

Published: 6 June 2007

Abstract

We constructed a restriction site associated DNA (RAD) marker microarray to facilitate rapid genetic mapping of zebrafish mutations. Using these microarrays with a bulk segregant approach, we localized previously unmapped mutations to genomic regions just a few centiMorgans in length. Furthermore, we developed an approach to assay individual RAD markers in pooled populations and refined one region. The RAD approach is highly effective for genetic mapping in zebrafish and is an attractive option for mapping in other organisms.