Figure 1.

Zebrafish RAD marker microarray construction. (a) EcoRI restriction site associated DNA (RAD) tag samples were isolated from the AB and WIK strains. (b) Clone libraries were generated that were enriched for RAD tags specific to AB or WIK, and these libraries were used to produce a RAD marker microarray. (c) AB and WIK RAD tag samples were fluorescently labeled and hybridized directly against each other to the microarray. Elements derived from a WIK-specific RAD tag should have differential hybridization associated with the WIK RAD tag sample. The same should be seen with AB-specific RAD tag elements and the AB RAD tag sample.