Table 2

b1166 RAD microarray mapping data
Marker name
 Experiment A: b1166 (wild type)/b1166 (mutant)
 Experiment B: WIK/AB
 Experiment A/experiment B
 Zv6 location
 Completely associated (RAD Amp)
WIK18I13
 5.78
 8.91
 0.65
 Chr:25 (23.7 Mb)
 No
WIK13P08
 5.12
 5.35
 0.96
 Chr:25 (12.0 Mb)
 No
WIK20J21
 4.97
 2.78
 1.78
 Chr:25 (24.9 Mb)
 Yes
WIK08N22
 4.57
 3.60
 1.27
 Chr:9 (30.9 Mb)
 n.t.
WIK05E18
 4.21
 4.87
 0.86
 Chr:25 (16.6 Mb)
 No
WIK18C01
 3.71
 3.73
 0.99
 Chr:25 (18.6 Mb)
 Yes
WIK11I12
 3.57
 3.09
 1.16
 Chr:25 (18.2 Mb)
 Yes
WIK07C02
 3.56
 3.51
 1.01
 Chr:25 (22.7 Mb)
 No
WIK07B09
 3.46
 3.22
 1.08
 Chr:21 (50.0 Mb)
 n.t.
WIK02K07
 3.06
 4.26
 0.72
 Chr:1 (17.9 Mb)
 n.t.

Normalized ratios are shown for microarray experiments. Experiment A is the hybridization of b1166 wild-type pool restriction site associated DNA (RAD) tags verses b1166 mutant pool RAD tags. Experiment B is the hybridization of AB RAD tags verses WIK RAD tags. The marker locations according to Zv6 assembly of the zebrafish genome are shown. In the 'Completely associated (RAD Amp)' column, entries are as follows: 'Yes' for RAD markers for which there was no wild-type allele in the b1166 mutant pool according to RAD Amp; 'No' for RAD markers for which there was wild-type allele present in the b1166 mutant pool according to RAD Amp; and 'n.t.' for RAD markers that were not tested using RAD Amp. RAD markers that are completely associated with the mutation often have higher ratios of experiment A to experiment B.

Miller et al. Genome Biology 2007 8:R105   doi:10.1186/gb-2007-8-6-r105

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