Figure 1.

Histological analysis of epidermis samples. (a) Hematoxylin-eosin stained sections of entire epidermis after thermolysin incubation and removal of the dermis. (b,c,d) Epidermis fragments remaining after the first, second, and third trypsin incubation, respectively. Fragments shown in (d) are mainly composed of GKs attached to the cornified layer and constitute the T4 fraction. Inset: higher magnification showing the characteristic cytological aspect of a GK with cytoplasmic keratohyalin granules.