Expression profile of newly identified genes. PCR experiments were performed with a commercial panel of cDNAs from 16 human tissues (PBL, peripheral blood leukocytes) and with cDNAs prepared from the T4 fraction enriched in GKs. For each gene, PCR primers were chosen to amplify a cDNA fragment encompassing at least two exons. Note the highly specific expression pattern of FLG2, LIPK, M, N, and, to a lesser extent, SERPINA12 genes. The apparent size variation of the CLDN23 fragment results from an artifactual gel distortion. Expression of GAPDH, assessed with the primers provided by the manufacturer, was used as a control.
Toulza et al. Genome Biology 2007 8:R107 doi:10.1186/gb-2007-8-6-r107