Figure 6.

C1orf81 mRNA expression and conservation of the eighth exon among mammals. (a) Expression pattern of the C1orf81 gene. PCR was performed with a commercial panel of cDNAs from 16 human tissues (PBL, peripheral blood leukocytes) and with cDNA prepared from epidermis. The amplified fragment (120 nt) encompasses exons 13-14. (b) Sequence alignment of the eighth exon of the C1orf81 gene from 11 mammals. The sequences were retrieved from the multiz17way table of the UCSC Genome Browser [73], and from a BLAST search of the cat genome. The consensus splicing signals are boxed. The black arrow indicates the single nucleotide insertion in the human gene. The alignment was created with Multalin software [71]. Asterisks indicate the positions conserved in all the sequences. The colors correspond to various levels of consensus, with red for high consensus and grey for low consensus.