Table 2

Proteins identified in both growth phases with their bioinformatic analysis and emPAI calculation
Accession no. (NCBI)
 Protein
 Mowse
 PSortB
 SignalP
 SecP
 emPAI
 Protein (M%)
 Fold change
 Species








0.3
 1.2
 L
 SP
 SP
0.3
 1.2
 0.3
 1.2

17985267
 60 kDa chaperonin GroEl
 1334
 1734
 C
 No
 No
 No
 0.778
 0.884
 3.068
 2.985
 1.0
 Bm
17982679
 Protein translation elongation factor Tu
 828
 1133
 C
 No
 AMA-KS17,18
 No
 0.897
 1.153
 3.537
 3.893
 0.9
 Bm
17982693
 Protein translation elongation factor G
 547
 884
 C
 No
 No
 No
 0.459
 0.503
 1.810
 1.698
 1.1
 Bm
17982686
 DNA directed RNA polymerase beta chain
 601
 686
 C
 No
 No
 No
 0.211
 0.183
 0.832
 0.618
 1.3
 Bm
17982688
 DNA directed RNA polymerase beta' chain
 461
 675
 C
 No
 No
 No
 0.132
 0.172
 0.520
 0.581
 0.9
 Bm
17984056
 DNAK protein (HSP 70)
 404
 613
 C
 No
 No
 Y 0.69
 0.225
 0.288
 0.887
 0.972
 0.9
 Bm
17982961
 30S ribosomal protein S1
 541
 611
 U
 No
 No
 Y 0.85
 4.623
 3.645
 18.228
 12.308
 1.5
 Bm
17983895
 Aconitate hydratase
 288
 563
 C
 No
 No
 No
 0.18
 0.297
 0.710
 1.033
 0.7
 Bm
17981970
 Electron transfer flavoprotein beta subunit
 396
 342
 U
 No
 No
 Y 0.63
 0.469
 0.469
 1.849
 1.584
 1.2
 Bm
17982110
 Membrane-bound lytic murien transglycosylase B
 238
 103
 CM
 No
 No
 No
 0.469
 0.202
 1.849
 0.682
 2.7
 Bm
17984018
 N utilization protein NusA
 75
 135
 C
 No
 No
 No
 0.096
 0.167
 0.379
 0.564
 0.7
 Bm
17982394
 Ribose-phosphate pyrophosphokinase
 95
 192
 U
 No
 No
 No
 0.146
 0.250
 0.576
 0.844
 0.7
 Bm
17982015
 Malate dehydrogenase
 174
 409
 C
 No
 TLA-HL25,26
 No
 0.291
 0.816
 1.147
 2.755
 0.4
 Bm
17982340
 Periplasmic dipeptide transport protein pre
 323
 371
 P
 No
 ASA-KT37,38
 Y 0.93
 0.39
 0.51
 1.538
 1.722
 0.9
 Bm
17982978
 Fumarate hydratase class I aerobic
 301
 309
 C
 No
 No
 No
 0.406
 0.291
 1.601
 0.983
 1.6
 Bm
17982732
 Isocitrate dehydrogenase (NADP)
 275
 396
 U
 No
 No
 No
 0.241
 0.333
 0.950
 1.124
 0.8
 Bm
17982121
 Phosphoribosylaminoimidazolecarboxamide formyltransferase
 261
 365
 C
 No
 No
 No
 0.216
 0.315
 0.852
 1.064
 0.8
 Bm
17983182
 Aspartyl-tRNA synthetase
 262
 334
 C
 No
 No
 No
 0.156
 0.197
 0.615
 0.665
 0.9
 Bm
17982205
 Transketolase
 252
 213
 C
 No
 KAA-DG16,17
 No
 0.222
 0.143
 0.875
 0.483
 1.8
 Bm
17982204
 Glyceraldehyde 3-phosphate dehydrogenase
 230
 288
 C
 No
 No
 No
 0.291
 0.377
 1.147
 1.273
 0.9
 Bm
17983520
 Enoyl-(acyl carrier protein) reductase (NADH)
 232
 216
 C
 No
 No
 No
 0.648
 0.493
 2.555
 1.665
 1.5
 Bm
17984008
 Enoyl-(acyl carrier protein) reductase (NADH)
 202
 197
 C
 No
 No
 No
 0.422
 0.556
 1.664
 1.877
 0.9
 Bm
17982437
 Carbamoyl-phosphate synthase large chain
 125
 286
 U
 Yes
 No
 No
 0.038
 0.161
 0.150
 0.544
 0.3
 Bm
17983107
 30S ribosomal protein S4
 206
 62
 U
 No
 No
 Y 0.54
 0.358
 0.107
 1.412
 0.361
 3.9
 Bm
17982692
 30S ribosomal protein S7
 81
 225
 U
 No
 No
 No
 0.167
 0.358
 0.658
 1.209
 0.5
 Bm
17985266
 10 kDa chaperonin GroES
 192
 168
 C
 No
 No
 No
 0.368
 0.368
 1.451
 1.243
 1.2
 Bm
23463995
 Conserved hypothetical protein
 94
 225
 C
 No
 No
 No
 0.146
 0.403
 0.576
 1.361
 0.4
 Bs
86279873
 Polyribonucleotide nucleotidyltransferase protein
 190
 146
 C
 No
 No
 No
 0.114
 0.114
 0.450
 0.385
 1.2
 Re
17983037
 Trigger factor, peptidylprolyl isomerase
 131
 224
 C
 No
 No
 No
 0.089
 0.119
 0.351
 0.408
 0.9
 Bm
17982138
 ATP synthase F1, alpha chain
 158
 112
 U
 No
 No
 No
 2.63
 2.63
 10.370
 8.880
 1.2
 Bm
17982141
 ATP synthase F1, beta chain
 180
 173
 U
 No
 AEA-KP15,16
 No
 0.233
 0.169
 0.919
 0.571
 1.6
 Bm
17984734
 Glycine dehydrogenase (decarboxylating)
 99
 218
 C
 No
 No
 No
 0.064
 0.127
 0.252
 0.429
 0.6
 Bm
17982133
 Transaldolase
 179
 218
 U
 No
 No
 No
 0.374
 0.374
 1.475
 1.263
 1.2
 Bm
17982713
 30S ribosomal protein S5
 164
 133
 C
 No
 No
 No
 0.138
 0.089
 0.544
 0.301
 1.8
 Bm
17982705
 30S ribosomal protein S17
 96
 205
 U
 No
 No
 Y 0.73
 0.025
 0.374
 0.099
 1.263
 0.1
 Bm
17983483
 Malonyl coa-acyl carrier protein transacylase
 160
 135
 C
 No
 No
 No
 0.259
 0.259
 1.021
 0.875
 1.2
 Bm
17982471
 ABC transporter ATP-binding protein YjjK
 84
 185
 CM
 No
 No
 No
 0.084
 0.114
 0.331
 0.385
 0.9
 Bm
17984086
 Adenosylhomocysteinase
 158
 159
 C
 No
 No
 No
 0.161
 0.161
 0.635
 0.544
 1.2
 Bm
17983095
 Phosphoribosylaminoimidazole-succinocarboxamide synthase
 157
 166
 C
 No
 No
 No
 0.321
 0.23
 1.266
 0.777
 1.6
 Bm
17982017
 Succinyl-CoA synthetase alpha chain
 155
 176
 C
 No
 No
 No
 0.291
 0.291
 1.147
 0.983
 1.2
 Bm
17982721
 DNA directed RNA polymerase alpha chain
 134
 65
 C
 No
 No
 No
 0.211
 0.138
 0.832
 0.466
 1.8
 Bm
17982016
 Succinyl-CoA synthetase beta chain
 76
 157
 C
 No
 No
 No
 0.094
 0.197
 0.371
 0.665
 0.6
 Bm
17983100
 Phosphoribosylformylglycinamidine synthase II
 119
 150
 U
 No
 No
 No
 0.13
 0.13
 0.513
 0.439
 1.2
 Bm
17982700
 30S ribosomal protein S19
 92
 150
 U
 No
 No
 Y 0.9
 0.291
 0.469
 1.147
 1.584
 0.7
 Bm
17983486
 30S ribosomal protein S18
 119
 63
 U
 No
 No
 Y 0.53
 0.197
 0.091
 0.777
 0.307
 2.5
 Bm
17982938
 Glutamine synthetase I
 115
 63
 C
 No
 No
 Y 0.63
 0.104
 0.104
 0.410
 0.351
 1.2
 Bm
23463708
 GMP synthase (glutamine-hydrolyzing)
 113
 87
 C
 No
 No
 No
 0.227
 0.107
 0.895
 0.361
 2.5
 Bs
17982702
 30S ribosomal protein S3
 57
 136
 C
 No
 No
 No
 0.045
 0.143
 0.177
 0.483
 0.4
 Bm
17982781
 Citrate synthase
 112
 114
 C
 No
 No
 No
 0.146
 0.146
 0.576
 0.493
 1.2
 Bm
17983059
 Arginyl-tRNA synthetase
 111
 113
 C
 No
 No
 No
 0.057
 0.057
 0.225
 0.192
 1.2
 Bm
17982196
 Hypothetical cytosolic protein
 102
 73
 U
 No
 No
 Y 0.69
 0.14
 0.069
 0.552
 0.233
 2.4
 Bm
17982768
 Protein translation elongation factor Ts
 99
 132
 C
 No
 No
 No
 0.067
 0.138
 0.264
 0.466
 0.6
 Bm
17983768
 Aldehyde dehydrogenase
 41
 111
 C
 No
 No
 No
 0.089
 0.138
 0.351
 0.466
 0.8
 Bm
17982113
 Chorismate mutase
 76
 82
 U
 No
 No
 No
 0.39
 0.39
 1.538
 1.317
 1.2
 Bm
17983157
 Integration host factor alpha subunit
 57
 102
 U
 No
 No
 Y 0.51
 0.089
 0.186
 0.351
 0.628
 0.6
 Bm

Cellular localizations: C, cytoplasmic; CM, cytoplasmic membrane; E, extracellular; P, periplasmic; U, unknown. SecP, SecretomeP; SP, signal peptide. Species: Bm, Brucella melitensis; Bs, Brucella suis; Re, Rhizobium etli.

Graham et al. Genome Biology 2007 8:R110   doi:10.1186/gb-2007-8-6-r110