Table 3

Proteins identified in late growth phase with their bioinformatic analysis and emPAI calculation

Accession no. (NCBI)

Protein

Mowse

PSortB

SignalP

SecP

emPAI

Protein (M%)

Species


L

SP

SP


17984094

Phosphoenol pyruvate carboxylase (ATP)

468

U

No

No

No

0.300

1.013

Bm

17983911

Arginosuccinate synthase

313

C

No

No

No

0.276

0.932

Bm

17982698

50S ribosomal protein L23

273

U

No

No

Y 0.5

0.097

0.327

Bm

17983035

Glutamyl-tRNA(GLN) amidotransferase subunit B

263

C

No

No

No

0.167

0.557

Bm

17982203

Phosphoglycerate kinase

178

C

No

No

No

0.239

0.807

Bm

17984924

Periplasmic oligopeptide-binding protein precursor

176

P

No

No

Y 0.89

0.183

0.618

Bm

17982826

DNA-binding protein HU alpha

170

U

No

LVA-AV10,11

Y 0.95

0.469

1.584

Bm

17982691

30S ribosomal protein S12

169

U

No

No

Y 0.83

0.291

0.983

Bm

17982154

Leucine, isoleucine, valine, threonine and alanine binding protein

157

P

Yes

AWA-DV28,29

Y 0.95

0.194

0.655

Bm

17984006

3-Hydroxydecanoyl-(acyl-carrier-protein) dehydratase

149

C

No

No

No

0.626

2.114

Bm

17983192

General L-amino acid-binding periplasmic protein AAPJ precursor

132

P

Yes

ASA-DT24,25

Y 0.65

0.225

0.760

Bm

17984058

Phenylalanyl-tRNA synthetase beta chain

131

C

No

No

No

0.054

0.182

Bm

17984780

N-methylhydantoinase (ATP-hydrolising) 5-oxoprolinase(EC3.5.2.9)

125

C

No

No

No

0.081

0.274

Bm

17983089

Adenylosuccinate lyase

120

C

No

No

No

0.086

0.290

Bm

17983993

30S ribosomal protein S20

120

U

No

No

Y 0.58

0.161

0.544

Bm

17983794

Hypothetical protein

119

C

No

No

No

0.469

1.584

Bm

17983437

Pyruvate, phosphate dikinase

112

C

No

No

0.047

0.159

Bm

17982937

Nitrogen regulatory protein P-II

108

C

No

No

No

0.233

0.789

Bm

17984078

Thioredoxin C-1

108

C

No

No

Y 0.84

0.291

0.983

Bm

17983171

Serine hydroxymethyltransferase

103

C

No

No

No

0.167

0.564

Bm

17984416

2,3,4,5-Tetrahdropyridine-2-carboxylate N-succinyltransferase

101

C

No

No

No

0.122

0.412

Bm

17984012

30S ribosomal protein S15

95

U

No

No

Y 0.54

0.069

0.233

Bm

17983482

Short-chain dehydrogenase

92

C

No

No

No

0.194

0.655

Bm

49238135

3-Oxoacyl-(acyl carrierprotein) reductase

92

C

No

No

No

0.076

0.257

Bh

17982682

50S ribosomal protein L11

91

U

No

AGA-AN17,18

Y 0.95

0.194

0.655

Bm

17984753

Alkyl hyroperoxide reductase C22 protein

85

C

No

No

No

0.274

0.925

Bm

17982411

Cold shock protein CSPA

82

C

No

No

Y 0.81

0.584

1.972

Bm

17983290

Dihydrodipicolinate synthase

82

C

No

ITA-LV22,23

No

0.122

0.412

Bm

17982131

Leucyl-tRNA synthetase

77

C

No

No

No

0.023

0.078

Bm

86283673

Dipeptide ABC transporter, substrate binding

75

P

Yes

AFA-ET31,32

Y 0.91

0.072

0.243

Re

17982712

50s ribosomal protein L18

74

U

No

No

No

0.072

0.243

Bm

23347767

Valyl-tRNA synthetase

73

C

No

No

No

0.019

0.064

Bs

17982719

30S ribosomal protein S13

72

C

No

No

No

0.072

0.243

Bm

17983459

Thiol peroxidase

69

U

No

No

Y 0.89

0.122

0.412

Bm

17982531

Hypothtical cytosolic protein

68

C

No

No

No

0.186

0.628

Bm

17984569

Osmotically inducible protein C

68

U

No

No

Y 0.82

0.069

0.233

Bm

17981953

Histidinol-phosphate aminotransferase

66

C

No

No

No

0.067

0.226

Bm

15073728

Probable isoleucyl-tRNA synthetase protein

64

C

No

No

No

0.038

0.128

Sm

17984859

Glutamyl-tRNA(GLN) amidotransferase subunit A

63

U

No

No

No

0.109

0.368

Bm

17984521

Urocanate hydratase

57

U

No

No

No

0.069

0.233

Bm


Cellular localizations: C, cytoplasmic; CM, cytoplasmic membrane; E, extracellular; P, periplasmic; U, unknown. SecP, SecretomeP; SP, signal peptide. Species: Bm, Brucella melitensis; Bs, Brucella suis; Re, Rhizobium etli; Sm, Sinorhizobium meliloti.

Graham et al. Genome Biology 2007 8:R110   doi:10.1186/gb-2007-8-6-r110

Open Data