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LongSAGE profiling of nine human embryonic stem cell lines

Martin Hirst1 email, Allen Delaney1 email, Sean A Rogers1 email, Angelique Schnerch1 email, Deryck R Persaud1 email, Michael D O'Connor2 email, Thomas Zeng1 email, Michelle Moksa1 email, Keith Fichter1 email, Diana Mah1 email, Anne Go1 email, Ryan D Morin1 email, Agnes Baross1 email, Yongjun Zhao1 email, Jaswinder Khattra1 email, Anna-Liisa Prabhu1 email, Pawan Pandoh1 email, Helen McDonald1 email, Jennifer Asano1 email, Noreen Dhalla1 email, Kevin Ma1 email, Stephanie Lee1 email, Adrian Ally1 email, Neil Chahal1 email, Stephanie Menzies1 email, Asim Siddiqui1 email, Robert Holt1 email, Steven Jones1 email, Daniela S Gerhard3 email, James A Thomson4 email, Connie J Eaves2 email and Marco A Marra1 email

Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada, V5Z 1L3

Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada, V5Z 1L3

National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA

Wisconsin National Primate Research Centre and Department of Anatomy, School of Medicine, University of Wisconsin, Madison, Wisconsin 53715, USA

author email corresponding author email

Genome Biology 2007, 8:R113doi:10.1186/gb-2007-8-6-r113

Published: 14 June 2007

Subject areas: Development, Molecular biology, Genome studies

Abstract

To facilitate discovery of novel human embryonic stem cell (ESC) transcripts, we generated 2.5 million LongSAGE tags from 9 human ESC lines. Analysis of this data revealed that ESCs express proportionately more RNA binding proteins compared with terminally differentiated cells, and identified novel ESC transcripts, at least one of which may represent a marker of the pluripotent state.


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