Open Access Method

LongSAGE profiling of nine human embryonic stem cell lines

Martin Hirst1, Allen Delaney1, Sean A Rogers1, Angelique Schnerch1, Deryck R Persaud1, Michael D O'Connor2, Thomas Zeng1, Michelle Moksa1, Keith Fichter1, Diana Mah1, Anne Go1, Ryan D Morin1, Agnes Baross1, Yongjun Zhao1, Jaswinder Khattra1, Anna-Liisa Prabhu1, Pawan Pandoh1, Helen McDonald1, Jennifer Asano1, Noreen Dhalla1, Kevin Ma1, Stephanie Lee1, Adrian Ally1, Neil Chahal1, Stephanie Menzies1, Asim Siddiqui1, Robert Holt1, Steven Jones1, Daniela S Gerhard3, James A Thomson4, Connie J Eaves2 and Marco A Marra1*

Author Affiliations

1 Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada, V5Z 1L3

2 Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada, V5Z 1L3

3 National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA

4 Wisconsin National Primate Research Centre and Department of Anatomy, School of Medicine, University of Wisconsin, Madison, Wisconsin 53715, USA

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Genome Biology 2007, 8:R113  doi:10.1186/gb-2007-8-6-r113

Published: 14 June 2007

Abstract

To facilitate discovery of novel human embryonic stem cell (ESC) transcripts, we generated 2.5 million LongSAGE tags from 9 human ESC lines. Analysis of this data revealed that ESCs express proportionately more RNA binding proteins compared with terminally differentiated cells, and identified novel ESC transcripts, at least one of which may represent a marker of the pluripotent state.