Method
LongSAGE profiling of nine human embryonic stem cell lines
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* Corresponding author: Marco A Marra mmarra@bcgsc.ca
1 Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada, V5Z 1L3
2 Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada, V5Z 1L3
3 National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
4 Wisconsin National Primate Research Centre and Department of Anatomy, School of Medicine, University of Wisconsin, Madison, Wisconsin 53715, USA
Genome Biology 2007, 8:R113 doi:10.1186/gb-2007-8-6-r113
Published: 14 June 2007Abstract
To facilitate discovery of novel human embryonic stem cell (ESC) transcripts, we generated 2.5 million LongSAGE tags from 9 human ESC lines. Analysis of this data revealed that ESCs express proportionately more RNA binding proteins compared with terminally differentiated cells, and identified novel ESC transcripts, at least one of which may represent a marker of the pluripotent state.