Genome Biology

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Open Access Method

LongSAGE profiling of nine human embryonic stem cell lines

Martin Hirst1, Allen Delaney1, Sean A Rogers1, Angelique Schnerch1, Deryck R Persaud1, Michael D O'Connor2, Thomas Zeng1, Michelle Moksa1, Keith Fichter1, Diana Mah1, Anne Go1, Ryan D Morin1, Agnes Baross1, Yongjun Zhao1, Jaswinder Khattra1, Anna-Liisa Prabhu1, Pawan Pandoh1, Helen McDonald1, Jennifer Asano1, Noreen Dhalla1, Kevin Ma1, Stephanie Lee1, Adrian Ally1, Neil Chahal1, Stephanie Menzies1, Asim Siddiqui1, Robert Holt1, Steven Jones1, Daniela S Gerhard3, James A Thomson4, Connie J Eaves2 and Marco A Marra1*

Author Affiliations

1 Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada, V5Z 1L3

2 Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada, V5Z 1L3

3 National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA

4 Wisconsin National Primate Research Centre and Department of Anatomy, School of Medicine, University of Wisconsin, Madison, Wisconsin 53715, USA

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Genome Biology 2007, 8:R113 doi:10.1186/gb-2007-8-6-r113

Published: 14 June 2007

Additional files

Additional data file 1:

Summary of mouse specific tag types identified.

Format: XLS Size: 17KB Download file

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Additional data file 2:

Genomic mappings for 268,515 unique tag sequences found in nine independent human embryonic stem cell lines.

Format: ZIP Size: 7.6MB Download file

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Additional data file 3:

Tag counts are expressed for each GO category for the top 1,000 by tag count.

Format: XLS Size: 22KB Download file

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Additional data file 4:

Statistically significant differentially expressed LongSAGE tags found between embryonic stem cells and terminally differentiated tissues.

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Additional data file 5:

The 4,337 genes found in common across 8 undifferentiated human embryonic stem cell lines.

Format: XLS Size: 211KB Download file

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Additional data file 6:

The 20,047 LongSAGE tags exclusively expressed in embryonic stem cell lines.

Format: XLS Size: 2.1MB Download file

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Additional data file 7:

The 634 LongSAGE tags exclusively expressed in ESCs that uniquely map to the human genome at least 2 kb away from an annotated transcript.

Format: XLS Size: 156KB Download file

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Additional data file 8:

The 301 LongSAGE tags exclusively expressed in ESCs that uniquely map to species conserved regions of the human genome at least 2 kb away from an annotated transcript.

Format: XLS Size: 73KB Download file

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Additional data file 9:

The 52 ESC specific transcripts identified by 5' RACE.

Format: XLS Size: 84KB Download file

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Additional data file 10:

RACE and qPCR primer sequences used in this study.

Format: XLS Size: 37KB Download file

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Open Data