Figure 2.

Coverage of the MGC by ESC sequence tag-based transcriptomes. Unambiguous tags from published MPSS and short SAGE ESC transcriptomes were mapped to genes in the MGC and compared to identically treated LongSAGE transcriptomes. To assess the impact of tag number on gene identification, the proportion of MGC sequences detected was plotted against increasing numbers of tags. Coverage of the MGC increases with increased numbers of tags for ESC LongSAGE libraries even at levels above 200,000 tags. In contrast, coverage of the MGC by the MPSS library plateaus early with little increase in coverage observed with increased sampling depth (MPSS). Coverage of the MGC by the short SAGE ESC libraries (ES03_SAGE and ES04_SAGE) is significantly lower due to the presence of ambiguous tags.