Table 6 |
||||
|
Primer sequences and PCR product sizes of genes selected for validation by QPCR |
||||
|
Gene |
GenBank ID |
Primer sequence |
Annealing Tm (°C) |
Product size (bp) |
|
|
||||
|
H3F3A |
Forward 5'-GCAAGAGTGCGCCCTCTACT-3' Reverse 5'-TTGGCATAATTGTTACACGTTTGG-3' |
60 |
288 |
|
|
TNNC2 |
Forward 5'-AAGGAGTTGGGCACCGTGAT-3' Reverse 5'-CGGCCTTCGTTGTTCTTGTC-3' |
60 |
326 |
|
|
MYL2 |
Forward 5'-GGGTGCTCAGGGCTGATTAT-3' Reverse 5'-AGGCTGCAAAGAAGATGAAGGT-3' |
60 |
326 |
|
|
MYL1 |
Forward 5'-GACTTTGTTGAGGGTCTGCG-3' Reverse 5'-GAGTGGTGCTTGGATTTGAG-3' |
60 |
452 |
|
|
SLN |
Forward 5'-AGAATGGAGCGATCCACCCG-3' Reverse 5'-AAACACTTGGCAGCCCTTGA-3' |
60 |
300 |
|
|
MYLPF |
Forward 5'-GAGAAGGGCAGCGGCAGAAG-3' Reverse 5'-GTGCGTGATGACGTAGCAGATGTT-3' |
60 |
466 |
|
|
TOB1 |
Forward 5'-TTACCACTGCCACTTTCGCT-3' Reverse 5'-TTCTGCTTCAAGAGGTCATTCAC-3' |
61 |
129 |
|
|
CRABP1 |
Forward 5'-GTGTGAACGCCATGCTGAG-3' Reverse 5'-CGTCCGTCCACTGTCTCC-3' |
55 |
169 |
|
|
LGALS1 |
Forward 5'-GGTCGCCAGCAACCTGAATCTC-3' Reverse 5'-GTCTCCGTGCATGTCGAAGCG-3' |
58 |
151 |
|
|
GNB2L1 |
Forward 5'-GCTGGGACAAGCTGGTCAAGG-3' Reverse 5'-AGCACAGAGCCAGTAGCGATTG-3' |
58 |
245 |
|
|
TPT1 |
Forward 5'-GGCTGTTGGGATCGGATCTATC-3' Reverse 5'-AACAATGCCTCCGCTCCAAAG-3' |
55 |
150 |
|
|
RPS28 |
Forward 5'-GGCAGGACAGGTTCGCAGG-3' Reverse 5'-ATATCCAGGACCCAGCCACAAC-3' |
56 |
179 |
|
|
TncRNA |
Forward 5'-GACCGCTGTCGTCACTGTATG-3' Reverse 5'-AGCACTTGCCCAGCCCTAG-3' |
55 |
189 |
|
|
|
||||
|
Tang et al. Genome Biology 2007 8:R115 doi:10.1186/gb-2007-8-6-r115 |
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