Table 1 |
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|
Thirteen DHFR* insertion site clones and their response to methotrexate |
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| Name |
Chr. |
Chr. band |
Sequence1 (bp) |
Exp.2 |
IC-503 (nM) |
MTX4 (nM) |
Expression5 (%) |
No. of colonies6 |
Colonies screened7 |
DHFR* amplicon8 |
DHFR* gain9 |
|
|
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| 1M-34 |
9 |
q34.3 |
137,580,684 |
→ |
43 |
120 |
4,172 |
26.5 (8.50) |
10 |
4 |
0 |
| 1M-39 |
13 |
q32.1 |
96,707,451 |
← |
22 |
65 |
3,835 |
0.5 (1.00) |
0 |
0 |
0 |
| 1M-42 |
8 |
q22.3 |
102,162,871 |
← |
21 |
75 |
2,660 |
5.5 (3.25) |
15 |
13 |
2 |
| 1M-43 |
11 |
q13.1 |
65,526,684 |
→ |
22 |
65 |
1,828 |
13 (10.50) |
9 |
0 |
3 |
| 1M-45 |
11 |
q23.3 |
118,293,388 |
← |
70 |
200 |
5,329 |
2.5 (3.00) |
8 |
1 |
6 |
| 1M-57 |
3 |
q27.1 |
185,207,363 |
→ |
15 |
75 |
2,047 |
3.5 (3.75) |
12 |
3 |
8 |
| 1M-67 |
1 |
p34.3 |
39,533,783 |
→ |
31 |
90 |
2,306 |
0.5 (1.00) |
3 |
1 |
0 |
| 1M-72 |
2 |
q35 |
217,190,120 |
← |
16 |
65 |
5,077 |
1.0 (2.00) |
2 |
1 |
1 |
| 1M-73 |
12 |
p13.2 |
12,767,229 |
← |
20 |
65 |
3,116 |
0.5 (1.00) |
1 |
0 |
0 |
| 1M-75 |
22 |
q12.2 |
28,977,607 |
→ |
32 |
90 |
3,307 |
25.5 (12.75) |
3 |
1 |
1 |
| 1M-83 |
19 |
q13.2 |
45,618,743 |
→ |
14 |
65 |
756 |
1.0 (1.75) |
6 |
0 |
1 |
| 1M-84 |
17 |
q12 |
34,161,068 |
→ |
23 |
75 |
3,934 |
11.0 (11.00) |
4 |
0 |
0 |
| 1M-89 |
19 |
q13.33 |
53,682,371 |
→ |
25 |
75 |
4,288 |
2.0 (1.00) |
9 |
3 |
0 |
|
|
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|
1Position in the human genome sequence (UCSC Genome Browser, May 2004 freeze). 2Exp., Direction of DHFR* integration in the genome; arrow to the right indicates that the DHFR* coding sequence is integrated in the direction from the p-arm to the q-arm of the chromosome. 3Methotrexate concentration that causes 50% inhibition of the cell growth after 6 days. 4Methotrexate (MTX) concentration used for selection of resistant colonies. 5Expression of DHFR* measured indirectly by quantitative RT-PCR (EGFP expression normalized to GUSB expression, 2-(dCt) × 100). 6Median number (and interquartile range in parentheses) of resistant colonies per 10 cm plate after 28 days of methotrexate treatment. 7Number of independent resistant colonies screened by array CGH. 8Resistant colonies with amplification of the DHFR* integration region. 9Resistant colonies with low level copy number gain of the DHFR* integration region. Chr., chromosome. |
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|
Gajduskova et al. Genome Biology 2007 8:R120 doi:10.1186/gb-2007-8-6-r120 |
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