Figure 6.

Functional analysis of the BRP-1-Y46G5A.24-KEL-8 interaction network. RNAi was performed in triplicate in the presence or absence of 100 μM cadmium. (a) The effect of inhibiting kel-8 or Y46G5A.24 in wild-type (WT), mtl-2 null (mtl-2 (gk125)) or mek-1 null (mek-1(ks54)) C. elegans on nematode growth in the presence of 100 μM cadmium. (b) Effects of cadmium exposure on growth of brp-1 null C. elegans (brp-1 (ok1084)). C. elegans body length of the cadmium exposed population was normalized to the mean body length in the same RNAi treatment group not exposed to cadmium. Results are displayed as mean body length ± SE (n = 200-500).