Table 3

Examples of significantly enriched GO annotation for the list of morphine-responsive genes

Mouse strain
Morphine treatment
mRNA change
Enriched GO annotation (GO ID)
Number of genes
P
Example genes

C57BL/6J
Acute
+a
Regulation of apoptosis (0042981)
11
9.1 × e-06b
Tsc22d3, Pmaip1, and Plekhf1
129P3/J
Chronic
-
Nucleosome assembly (0006334)
5
1.7 × e-04
Hist1h1c, Hist1h3b, and Hist1h2bl
C57BL/6J
Chronic
-
Transmission of nerve impulse (0019226)
10
8.6 × e-04
Sncaip, Adora2a, and Npy5r
DBA/2J
Acute
+
Response to abiotic stimulus (0009628)
15
9.8 × e-04
Cryab, Dnajb1, and Cdkn1a
DBA/2J
Acute
+
Response to temperature stimulus (0009266)
5
1.7 × e-03
Hspa8, Hspa1b, and Cryab
C57BL/6J
Chronic
-
Cellular lipid metabolism (0044255)
17
1.95 × e-04
Vldr, Sc5d, and Acsl3
SWR/J
Acute
+
Glucose transport (0015758)
4
2.0 × e-03
Stxbp3a, Stxbp4, and Aps
SWR/J
Chronic
-
Cell communications (0007154)
25
2.3 × e-03
Plcd4, Gjb1, and Utrn
129P3/J
Acute
-
Neuron differentiation (0030182)
5
2.4 × e-03
Olig2, Gli3, and Ablim1
SWR/J
Acute
+
Carboxylic acid metabolism (0019752)
16
1.4 × e-03
Cpt1a, Cpt2, and Crot
C57BL/6J
Chronic
+
Negative regulation of biological process (0048519)
12
7.5 × e-03
Zbtb16, Sgk3, and Adrb1
SWR/J
Acute
-
Nervous system development (0007399)
7
9.9 × e-03
Olig2, Rgma, and Ugt8a
129P3/J
Chronic
+
Intracellular signaling cascade (0007242)
12
1.0 × e-02
Ppm1, Dab1, and Snx24

The complete results of the Gene Ontology (GO) analysis are presented in Additional data file 3. a(+) increased mRNA abundance in response to morphine; (-) decreased mRNA level. bStatistical significance of functional over-representation was computed using Fisher's exact test.

Korostynski et al. Genome Biology 2007 8:R128   doi:10.1186/gb-2007-8-6-r128