Additional data file 9.
Figure A shows a 15% PAGE separation of small-enriched RNAs from honey bee queen head, thorax, and abdomen, and worker head, thorax, and abdomen. RNA sized at 18-30 nt was excised from the gel and purified for qPCR as described in the text. The left lane shows a 10 nt RNA size marker, with the 10 nt band at bottom left. Figure B shows the size variation of PCR products generated from small-enriched RNA pools and candidate primers. Most products were approximately 75-90 bp in length. Alphanumeric label refers to sample ID as described in Additional data file 3.
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Weaver et al. Genome Biology 2007 8:R97 doi:10.1186/gb-2007-8-6-r97