|
Description of tested miRNAs |
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| Locus |
miRBase ID |
Primer ID |
Orientation |
Location |
Expression (not size selected) |
Expression (size selected) |
|
|
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| ame-mir-1 |
ame-mir-1 |
ame-mir-1.F |
F |
M |
0.06 |
N/A |
| ame-mir-1 |
ame-mir-1 |
amir1.F |
F |
P |
1.05 |
1.28 |
| ame-mir-124 |
ame-mir-124 |
miR-124M351 |
F |
M |
2.09 |
0.69 |
| ame-mir-124 |
ame-mir-124 |
miR-124M351R |
R |
M |
0.40 |
0.07 |
| ame-mir-2-1 |
ame-mir-2-1 |
ame-mir-2+.F |
F |
M |
7.81 |
13.55 |
| ame-mir-2-2 |
ame-mir-2-2 |
|||||
| ame-mir-2-3 |
ame-mir-2-3 |
|||||
| ame-mir-2-1 |
ame-mir-2-1 |
mir-2:1.1:101712.F |
F |
P |
0.06 |
0.02 |
| ame-mir-278 |
ame-mir-278 |
ame-mir-278.F |
F |
M |
0.37 |
0 |
| ame-mir-7 |
ame-mir-7 |
ame-mir-7.F |
F |
M |
17.95 |
3.16 |
| ame-mir-7 |
ame-mir-7 |
miR-7M112R |
F |
M |
0.06 |
N/A |
| ame-mir-9a |
ame-mir-9a |
ame-mir-9a.F |
F |
M |
23.69 |
9.58 |
| ame-mir-9b |
ame-mir-9b |
ame-mir-9b |
F |
M |
0.64 |
1.95 |
| ame-mir-iab-4 |
ame-mir-iab-4 |
ame-miriab4.F |
F |
P |
0.24 |
0.06 |
| ame-mir-10 |
ame-mir-10 |
ame-miR-10 |
F |
M |
0.09 |
N/A |
| ame-mir-279 |
ame-mir-279 |
miR-279M341 |
F |
M |
5.53 |
1.04 |
| ame-mir-279 |
ame-mir-279 |
miR-279M341R |
R |
M |
0.56 |
N/A |
| ame-mir-283 |
ame-mir-283 |
HCmir-283.F |
F |
M |
23.69 |
17.88 |
| ame-mir-71 |
ame-mir-71 |
miR-71.R |
R |
M |
1.70 |
1.38 |
| ame-mir-87-2 |
ame-mir-87-2 |
mir-87:13.12:403730.F |
F |
P |
0.43 |
0.91 |
| ame-bantam |
ame-bantam |
banM365 |
F |
M |
0.16 |
0.06 |
| C1504 |
ame-mir-925 |
C1504.F |
F |
M |
0.11 |
0.30 |
| C2989 |
ame-mir-926 |
C2989.F |
F |
M |
31.25 |
N/A |
| C3345 |
ame-mir-927 |
contig3345.R |
R |
M |
0.17 |
3.63 |
| C4222 |
ame-mir-928 |
C4222.F |
F |
M |
N/A |
3.39 |
| C5152a |
ame-mir-190* |
C5152a.F |
F |
O3 |
0.52 |
0.64 |
| C5152a |
ame-mir-190* |
D+ |
R |
O |
1.29 |
0.20 |
| C5152b |
ame-mir-190 |
C5152b.F |
F |
P |
0 |
0 |
| C5152b |
ame-mir-190 |
F- |
R |
P |
76.96 |
25.28 |
| C5303 |
ame-mir-137 |
C5303.F |
F |
O3 |
2.96 |
0.74 |
| C5303 |
ame-mir-137 |
C+ |
R |
O3 |
0.15 |
0.02 |
| C5560 |
ame-mir-929 |
C5560.F |
F |
O |
955,568 |
45,073 |
| C5560 |
ame-mir-929 |
A- |
R |
O3 |
0.01 |
0 |
| C5560 |
ame-mir-929 |
C5560.R |
R |
M |
0.00 |
0 |
| C5599 |
ame-mir-930 |
C5599b.F |
F |
M |
0.54 |
0.08 |
| C689 |
ame-mir-932 |
amir1.R |
F |
M |
0.23 |
0.28 |
| C689 |
ame-mir-932 |
contig689.F |
F |
P |
20.62 |
N/A |
| C689 |
ame-mir-932 |
C689.F |
R |
M |
0.16 |
N/A |
| C689 |
ame-mir-932 |
E+ |
R |
M |
N/A |
1.69 |
| C2187† |
C2187.F |
F |
M |
0.98 |
N/A |
|
| C2370† |
C2370.F |
F |
M |
8.37 |
N/A |
|
| C5581a‡ |
C5581a.R |
R |
M |
20.62 |
N/A |
|
| C5581b‡ |
C5581b.F |
F |
M |
0.07 |
0 |
|
| C5581b‡ |
C5581b.R |
R |
M |
22.10 |
N/A |
|
|
Orientation is on predicted miRNA (F, forward; R, reverse). Location is within: mature miRNA (M); precursor sequences (P); overlapping mature miRNA with 3' primer end within mature sequence (O); overlapping mature miRNA but with 3' primer end in precursor (O3). Expression levels for pooled queen and worker samples are described in the text. The last two columns are normalized expression estimates for pooled RNA that either had or had not been size-selected by PAGE to include sizes from 18-30 nt. *C5152a is the reverse complement of ame-mir-190. †C2187 and C2370 met thermodynamic criteria, but did not meet miRBase folding criteria. ‡Denotes U4 spliceosome RNA. The expression levels are scaled to the average of all primers. | ||||||
Weaver et al. Genome Biology 2007 8:R97 doi:10.1186/gb-2007-8-6-r97 |
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