Table 1

Number of bimodally expressed genes

Sample
Modela
Line effectb
Bimodalc

NC
Raw data
297
206

Mixed model normalization
192
324

Loess normalization
470
304

Both mixed and loess
188
162
CA
Raw data
285
243

Mixed model normalization
119
409

Loess normalization
406
319

Both mixed and loess
114
131
Common to both CA and NC

204
69d

a'Raw data' refers to analysis directly on the log transformed raw fluorescence intensity measures, without normalization to remove array effects. 'Mixed model' refers to gene-specific models after mixed model normalization (as described in Materials and methods). 'Loess normalization' refers to analysis after loess treatment of the arrays. Note that loess increases the number of genes with significant line effects, but it reduces the number with apparent bimodality. bThe number of genes exhibiting greater line variation than the residual when treating the line effect as a random factor. cThe number of genes for which the mixture modeling indicates a greater likelihood that the distribution of transcript abundance across lines has two or more modes. dThe total number of genes with bimodal expression in both populations, either from the mixed (48 genes), loess (33 genes), or both modes of analysis (12 genes). CA, California, NC, North Carolina.

Hsieh et al. Genome Biology 2007 8:R98   doi:10.1186/gb-2007-8-6-r98