Open Access Research

Extensive genomic diversity and selective conservation of virulence-determinants in enterohemorrhagic Escherichia coli strains of O157 and non-O157 serotypes

Yoshitoshi Ogura12, Tadasuke Ooka2, Asadulghani2, Jun Terajima3, Jean-Philippe Nougayrède4, Ken Kurokawa5, Kousuke Tashiro6, Toru Tobe7, Keisuke Nakayama2, Satoru Kuhara6, Eric Oswald4, Haruo Watanabe3 and Tetsuya Hayashi12*

Author Affiliations

1 Division of Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki,5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan

2 Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki,5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan

3 Department of Bacteriology, National Institute for Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan

4 UMR1225, INRA-ENVT, 23 chemin des Capelles, 31076 Toulouse, France

5 Laboratory of Comparative Genomics, Graduate School of Information Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan

6 Laboratory of Molecular Gene Technics, Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakosaki, Fukuoka, 812-8581, Japan

7 Division of Applied Bacteriology, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan

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Genome Biology 2007, 8:R138  doi:10.1186/gb-2007-8-7-r138

Published: 10 July 2007

Additional files

Additional data file 1:

XbaI-digestion patterns of EHEC genomic DNA are shown.

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Additional data file 2:

The MLST analysis of EHEC strains of the present study with other pathogenic E. coli strains in the EcMLST database was conducted by using concatenated DNA sequences of seven loci (aspC, clpX, fadD, icdA, lysP, mdh and uidA). The sequences of reference strains were obtained from EcMLST. Multiple sequence alignments were made by using the ClustalW program in the MEGA3 software. The NJ tree was generated by using the Tamura-Nei evolutionary model. Bootstrap values greater than 50% are indicated. The scale bar represents the number of substitutions per site. Accession numbers in EcMLST, strain names, serotypes, and classes in EcMLST of each strain are indicated. MLST20 is an undefined clonal group.

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Additional data file 3:

Plasmid profiles of O157 and non-O157 EHEC strains are shown.

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Additional data file 4:

The results of CGH analyses of O157 and non-O157 EHEC strains are summarized.

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Additional data file 5:

Conservation of the 'conserved in K-12' singleton genes belonging to each COG category was analyzed in each EHEC serotype.

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Additional data file 6:

The processed data of CGH analyses of non-O157 EHEC strains are shown.

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Open Data