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Ogura Y
Ooka T
Terajima J
Nougayrède JP
Kurokawa K
Tashiro K
Tobe T
Nakayama K
Kuhara S
Oswald E
Watanabe H
Hayashi T

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Ogura Y
Ooka T
Terajima J
Nougayrède JP
Kurokawa K
Tashiro K
Tobe T
Nakayama K
Kuhara S
Oswald E
Watanabe H
Hayashi T
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Research

Extensive genomic diversity and selective conservation of virulence-determinants in enterohemorrhagic Escherichia coli strains of O157 and non-O157 serotypes

Yoshitoshi Ogura¹^,2 , Tadasuke Ooka² , Asadulghani² , Jun Terajima³ , Jean-Philippe Nougayrède⁴ , Ken Kurokawa⁵ , Kousuke Tashiro⁶ , Toru Tobe⁷ , Keisuke Nakayama² , Satoru Kuhara⁶ , Eric Oswald⁴ , Haruo Watanabe³ and Tetsuya Hayashi¹^,2

¹Division of Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki,5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan

²Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki,5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan

³Department of Bacteriology, National Institute for Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan

⁴UMR1225, INRA-ENVT, 23 chemin des Capelles, 31076 Toulouse, France

⁵Laboratory of Comparative Genomics, Graduate School of Information Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan

⁶Laboratory of Molecular Gene Technics, Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakosaki, Fukuoka, 812-8581, Japan

⁷Division of Applied Bacteriology, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan

author email corresponding author email

Genome Biology 2007, 8:R138doi:10.1186/gb-2007-8-7-r138


Published:	10 July 2007

Subject areas: Evolution, Genome studies, Microbiology and parasitology, Bioinformatics

Additional files

Additional data file 1:

XbaI-digestion patterns of EHEC genomic DNA are shown.

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Additional data file 2:

The MLST analysis of EHEC strains of the present study with other pathogenic E. coli strains in the EcMLST database was conducted by using concatenated DNA sequences of seven loci (aspC, clpX, fadD, icdA, lysP, mdh and uidA). The sequences of reference strains were obtained from EcMLST. Multiple sequence alignments were made by using the ClustalW program in the MEGA3 software. The NJ tree was generated by using the Tamura-Nei evolutionary model. Bootstrap values greater than 50% are indicated. The scale bar represents the number of substitutions per site. Accession numbers in EcMLST, strain names, serotypes, and classes in EcMLST of each strain are indicated. MLST20 is an undefined clonal group.

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Additional data file 3:

Plasmid profiles of O157 and non-O157 EHEC strains are shown.

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Additional data file 4:

The results of CGH analyses of O157 and non-O157 EHEC strains are summarized.

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Additional data file 5:

Conservation of the 'conserved in K-12' singleton genes belonging to each COG category was analyzed in each EHEC serotype.

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Additional data file 6:

The processed data of CGH analyses of non-O157 EHEC strains are shown.

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