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Open Access Highly Accessed Research

Accuracy and quality of massively parallel DNA pyrosequencing

Susan M Huse, Julie A Huber, Hilary G Morrison, Mitchell L Sogin* and David Mark Welch

Author Affiliations

Josephine Bay Paul Center, Marine Biological Laboratory at Woods Hole, MBL Street, Woods Hole, MA 02543, USA

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Genome Biology 2007, 8:R143  doi:10.1186/gb-2007-8-7-r143

Published: 20 July 2007

Additional files

Additional data file 1:

The 43 known sequences used

Format: FA Size: 5KB Download file

Open Data

Additional data file 2:

These sequences correspond to those included in Additional data files 3-5 but include only the final sequence information in fasta format.

Format: GZ Size: 3.3MB Download file

Open Data

Additional data file 3:

Text translation of the original GS20 binary output (sff) file for the first of three PicoTiterPlate™ regions used in the analysis.

Format: ZIP Size: 36.6MB Download file

Open Data

Additional data file 4:

Text translation of the original GS20 binary output (sff) file for the second of three PicoTiterPlate™ regions used in the analysis.

Format: ZIP Size: 37.9MB Download file

Open Data

Additional data file 5:

Text translation of the original GS20 binary output (sff) file for the third of three PicoTiterPlate™ regions used in the analysis.

Format: ZIP Size: 36.7MB Download file

Open Data