Accuracy and quality of massively parallel DNA pyrosequencing

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Susan M Huse , Julie A Huber , Hilary G Morrison , Mitchell L Sogin and David Mark Welch

Josephine Bay Paul Center, Marine Biological Laboratory at Woods Hole, MBL Street, Woods Hole, MA 02543, USA

author email corresponding author email

Genome Biology 2007, 8:R143doi:10.1186/gb-2007-8-7-r143


Published:	20 July 2007

Subject areas: Genome studies, Methods

Additional data file 1:

The 43 known sequences used

Format: FA Size: 5KB Download file

Additional data file 2:

These sequences correspond to those included in Additional data files 3-5 but include only the final sequence information in fasta format.

Format: GZ Size: 3.3MB Download file

Additional data file 3:

Text translation of the original GS20 binary output (sff) file for the first of three PicoTiterPlate™ regions used in the analysis.

Format: ZIP Size: 36.6MB Download file

Additional data file 4:

Text translation of the original GS20 binary output (sff) file for the second of three PicoTiterPlate™ regions used in the analysis.

Format: ZIP Size: 37.9MB Download file

Additional data file 5:

Text translation of the original GS20 binary output (sff) file for the third of three PicoTiterPlate™ regions used in the analysis.

Format: ZIP Size: 36.7MB Download file