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Phenotypic characters used in the analysis |
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| Annotated stage |
Wild-type features |
Examples of mutant features |
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| Growth |
Growth, attachment, cell size |
Slow growth, no attachment, large cells |
| Wave |
5 min periodicity terminates at 6-7 h after starvation |
Slow oscillations, rapid onset, early termination, |
| Aggregation |
Cell streaming with or without late break up |
Cell clumping, partial developmental arrest, early break up |
| Mound |
Round mounds giving rise to slugs |
Arrest, multiple tips, disintegrating mound |
| Slug |
Migration with a smooth persistent trajectory |
Slow migration, arrested migration |
| Fruiting body |
Wild-type culminant structure |
Short stalk, long stalk, other aberrant morphology |
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Phenotype was scored subjectively by comparison of qualitative features of the strain for each stage of development shown above against those of the parental wild-type AX4 strain. For each sample run, these characters were checked by eye from the movies and observation of the samples by microscopy. On the basis of these features, the phenotype matrix pij was obtained for further analyses (see Materials and methods). | ||
Sawai et al. Genome Biology 2007 8:R144 doi:10.1186/gb-2007-8-7-r144 |
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