Table 1

Systematic variation from consensus DR1-type PPRE
Percent binding strength
 PPRE position


1
 2
 3
 4
 5
 6
 7
 8
 9
 10
 11
 12
 13
PPARα
     Consensus (90-100)
 A/G
 G
 G
 T
 A/C
 A
 A
 A
 G
 G
 T
 C
 A
     Class I (60-90)
 T
C
G
T
 G
T
 C/G
 A/G
 G
     Class II (30-60)
 C
 T
 A/T
 A/C/G
 T
 T
 C/G
 C
 A/C/T

T
 C/T
     Class III (0-30)
A/C


C/G
T
A/C
 A















PPARγ
     Consensus (90-100)
 A/G
 G
 G
 T
 C/G
 A
 A
 A
 G
 G
 T
 C
 A
     Class I (60-90)


C/G
 A/T
T
 G
T
 C/G
 A/G/T
 G
     Class II (30-60)
 C/T
 A/T
 T
 A

C
 C
 A/C/T


C/T
     Class III (0-30)
C
 A/C

C/G/T
 G
 T
A/C
 A















PPARβ/δ
     Consensus (90-100)
 A/G
 G
 G
 T
 C
 A
 A
 A
 G
 G
 T
 C
 A
     Class I (60-90)


C/G
 G/T
T
 G
T
G/T
     Class II (30-60)
 C
 A/T
 T
 A
 A


A/T
C/G
 A
 G/C/T
     Class III (0-30)
 T
 C
 A/C

C/G/T
 C/G
 C/T
 C
 A/C
 A

The binding strengths of in vitro translated PPAR-RXR heterodimers to 39 systematic variations of the DR1-type consensus PPRE AGGTCAAAGGTCA were determined by gelshift assays in reference to this consensus PPRE. Based on their average binding strength, all variations are sorted into three classes.

Heinäniemi et al. Genome Biology 2007 8:R147   doi:10.1186/gb-2007-8-7-r147

Open Data