Table 2 |
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Genomic PCR primers |
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| Gene (region) |
Location |
Primer sequences (5'-3') |
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| ACOX1 (distal) |
-4919 to -4643 |
TGAGCTCTTGATCTCCTCCTCAGAGTCATAG GAGTCTAGACTGGCAATCTTAGCAGAGTTC |
| ACOX1 (published) |
-1646 to -1374 |
TGAGCTCTTGAACTAGAAGGTCAGCTGTC GGGTCTAGACTAGCCTGTCTGTAGTCTGTG |
| ACOX1 (intron 1) |
+599 to +716 |
TGAGCTCTTGTGATTCAGGGAGGGTGGAAC GGGTCTAGACTGGCTGCGAGTGAGGAAG |
| ACOX1 (intron 2) |
+2822 to +3154 |
TGAGCTCTTGAGATAGAGTAACTCCTCCTAG GAGTCTAGAGAAGTGTGTCAAAGGGTGTG |
| ANGPTL4 (distal) |
-6765 to -6535 |
TGAGCTCTTGAACTAGAAGGTCAGCTGTC GAGTCTAGAATACACTCATGCAGGGTGAGG |
| ANGPTL4 (cluster) |
+2829 to +3610 |
TGAGCTCTTCTCCGTTCATCTCGAACCAC GAGTCTAGACATCTCAGAGGCTCTGCCTG |
| APOC3 (distal 1) |
-6429 to -6143 |
TGAGCTCTTGCTCAGGCGATAGTTAGAAG GAGTCTAGACTGGATGGTCCCACTCCAGTG |
| APOC3 (distal 2) |
-4249 to -3886 |
TGAGCTCTTGACTATGAGGTGACATCCAGG GAGTCTAGAGGACACACAGGCAGTACGTG |
| APOC3 (proximal) |
-870 to -568 |
TGAGCTCTTGGCAGTGAGGGCTGCTCTTC GGGTCTAGACATCTCTGGGTTTCAATCCAG |
| APOC3 (published) |
-262 to -3 |
ATTTCTAGACAGTCAGCTAGGAAGGAATGAG GGGTCTAGACTAGGGATGAACTGAGCAGAC |
| APOC3 (intron) |
+2424 to +2722 |
TGAGCTCTTGATCACACAACTAATCAATCCTC GAGTCTAGACTCAACTTCACTGGACGACAG |
| APOA1 (proximal) |
+7701 to +8022 (relative to APOC3 TSS) |
TGAGCTCTTCCTTCTCGCAGTCTCTAAGC GAGTCTAGAGCCAACACAATGGACAATGG |
| CPTIB |
-306 to -64 |
ATTTCTAGACAGAGTCTCGTGAGGATGGTG GGGTCTAGAGTTAGCGTTCATGCTGCCAG |
| PPARa (distal) |
-1376 to -1156 |
TGAGCTCTTCTGGCTAACATGTGCAAGAG GGGTCTAGACACTGTGCTATTTGTGGCAG |
| PPARa(proximal) |
-938 to -634 |
TGAGCTCTTCTCCTTGCTCTGGCAGAGTC GGGTCTAGACTCAGAAGTGCGTAGGGTG |
| RVRa(distal) |
-7279 to -7040 |
TGAGCTCTTGACCTTCCCAAGCCAAGAAC GAGTCTAGACACTAACCTCACAGACCACTG |
| RVRa(proximal) |
-510 to -70 |
TGAGCTCTTCTGGAGGTGTTCTCCCTAAG GTGTCTAGACTGCGCAACGACAAGACTG |
| RVRa(TSS) |
-510 to +119 (subcloned -266 to +119) |
TGAGCTCTTCTGGAGGTGTTCTCCCTAAG GTGTCTAGATTTCACTCTGCCAATCTCAGC |
| SULT2A1 |
-6104 to -5797 |
ATTTCTAGACTTGAATGGAAATGCCTGCTC GGGTCTAGAGACTGGGAAGTGGGAGGAGT |
| UCP3 (distal) |
-9680 to -9349 |
TGAGCTCTTCTCTAGTCTAAGTGCCTTGTC GAGTCTAGAGTAACAGTGAGCCTCTGGTCTG |
| UCP3 (proximal) |
-396 to -89 |
TGAGCTCTTGTACCTATCTCATAGGATTGTG GTGTCTAGAGTTGACAGCCTGATCACTTGAC |
| UCP3 (cluster) |
+2036 to +2303 |
TGAGCTCTTCAGGACTATGGTTGGACTGAAG GGGTCTAGAGATGGGAGGAGGCAAGGAAG |
| UCP3 (intron) |
+5971 to +6236 |
TGAGCTCTTCTCGTGCTGAGCACTTTACAC GAGTCTAGACACTTGTTGGGTCCATTCTAAC |
| LASS1 (region 1) |
-5297 to -4917 |
TGAGCTCTTCTGATGTGCAATCTCAGACAG GAGTCTAGACTCAGTCTCCACCATGAAGG |
| LASS1 (region 2) |
-2819 to -2499 |
TGAGCTCTTCCTCCCAGATGTCACCATTG GAGTCTAGACCTCTTTTGCCACTTCCCTC |
| LASS1 (region 3) |
-1389 to -978 |
TGAGCTCTTGTGGAACAGGAGCCATAGAG GGGTCTAGACATCGAGGAAGACACTGGTC |
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Sequence and location of the primer pairs used for real-time PCR quantification of genomic regions containing putative REs within the nine PPAR target genes. The positions indicated are in relation to the respective annotated gene TSS. The same primers were used for subcloning; the gene-specific sequences are indicated in bold. |
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Heinäniemi et al. Genome Biology 2007 8:R147 doi:10.1186/gb-2007-8-7-r147 |
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