DEP and ox-PAPC induce HO-1 expression in HMEC. (a) Western blot. HMEC were treated with DEP, ox-PAPC or a combination of both at various concentrations. Mouse monoclonal anti-HO-1 and anti-β-actin antibodies were used to detect the relevant proteins as described in Materials and methods. (b) Densitometric analysis. The expression level of HO-1 protein in optical density (OD) units is shown. Similar levels of β-actin are shown in (a). Results are typical of one representative experiment (n = 4).
Gong et al. Genome Biology 2007 8:R149 doi:10.1186/gb-2007-8-7-r149