DEP and ox-PAPC induce a large number of genes in HMEC. (a) Experimental protocol. HMEC were treated in triplicate wells with DEP, ox-PAPC, or DEP + ox-PAPC at the various concentrations shown. Cells were harvested at 4 h and cytoplasmic RNA prepared. Illumina microarrays were run and the data confirmed by qPCR analysis of selected genes. (b) Venn diagrams of upregulated genes. The numbers of genes that were significantly upregulated (> 1.5-fold, p < 0.05) over controls (no treatment) by the various treatment are shown. The left Venn diagram summarizes the number of genes induced by DEP 5 μg/ml (DEP5), ox-PAPC 10 μg/ml (ox10) and DEP5 + ox10. The middle Venn diagram shows the number of genes induced by DEP5, ox-PAPC 20 μg/ml (ox20) and DEP5 + ox20. The right Venn diagram summarizes the number of genes induced by DEP5, ox-PAPC 40 μg/ml (ox40) and DEP5 + ox40. The total number of genes induced by a particular condition can be found by adding all values displayed within the circle corresponding to that condition. Values displayed in the circle intersections indicate the number of genes induced in common by the intersecting conditions.
Gong et al. Genome Biology 2007 8:R149 doi:10.1186/gb-2007-8-7-r149