Genome Biology

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Exon expression profiling reveals stimulus-mediated exon use in neural cells

Adrienne E McKee1, Nicola Neretti2,3, Luis E Carvalho4, Clifford A Meyer5,6, Edward A Fox7,8, Alexander S Brodsky10,9* and Pamela A Silver1*

Author Affiliations

1 Department of Systems Biology, 200 Longwood Avenue, Harvard Medical School, Boston, Massachusetts 02115, USA.

2 Department of Physics, Institute for Brain and Neural Systems, 182 Hope Street, Brown University, Providence, Rhode Island 02912, USA.

3 Center for Computational Molecular Biology, 115 Waterman Street, Brown University, Providence, Rhode Island 02912, USA.

4 Department of Applied Mathematics, 182 George Street, Brown University, Rhode Island 02912, USA.

5 Department of Biostatistics and Computational Biology, Dana-Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts 02115, USA.

6 Department of Biostatistics, Harvard School of Public Health, 655 Huntington Avenue, Boston, Massachusetts 02115, USA.

7 Department of Medicine, 200 Longwood Avenue, Harvard Medical School, Boston, Massachusetts 02115, USA.

8 Department of Medical Oncology, Dana-Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts 02115, USA.

9 Department of Molecular Biology, Cell Biology, and Biochemistry, 185 Meeting Street, Brown University, Providence, Rhode Island 02903, USA.

10 Center for Genomics & Proteomics, Center for Computational Molecular Biology, 70 Ship Street, Brown University, Providence, Rhode Island 02903, USA.

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Genome Biology 2007, 8:R159 doi:10.1186/gb-2007-8-8-r159

Published: 2 August 2007

Additional files

Additional data file 1:

Presented is a figure of PCR data confirming the stimulus-induced transcription of known mRNAs.

Format: PDF Size: 265KB Download file

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Additional data file 2:

Presented is a table containing the transcripts affected by elevated [Ca2+]i.

Format: TXT Size: 2.3MB Download file

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Additional data file 3:

Presented is a table containing the exons affected by elevated [Ca2+]i.

Format: ZIP Size: 6.8MB Download file

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Additional data file 4:

Presented is a figure presenting the distribution of the number of exons called as changing in the KCl datasets.

Format: PDF Size: 249KB Download file

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Additional data file 5:

Presented is a table listing the GO categories enriched among transcripts affected by elevated [Ca2+]i.

Format: PDF Size: 23KB Download file

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Additional data file 6:

Presented is a figure showing analyses of transcript and exon PCR validation.

Format: PDF Size: 405KB Download file

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Additional data file 7:

Presented is a figure showing quantification of transcript PCR validation.

Format: PDF Size: 273KB Download file

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Additional data file 8:

Presented is a table listing the GO categories enriched among transcripts affected at the exon level by elevated [Ca2+]i through KCl treatment.

Format: PDF Size: 33KB Download file

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Additional data file 9:

Presented is a figure showing examples of splice form diversity identified from array data and verified by PCR.

Format: PDF Size: 310KB Download file

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Additional data file 10:

Presented is a table of PCR primers used in this study.

Format: XLS Size: 57KB Download file

This file can be viewed with: Microsoft Excel Viewer

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