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Microarray-based gene expression profiles in multiple tissues of the domesticated silkworm, Bombyx mori

Qingyou Xia1, Daojun Cheng1, Jun Duan1, Genhong Wang1, Tingcai Cheng1, Xingfu Zha1, Chun Liu1, Ping Zhao1, Fangyin Dai1, Ze Zhang1, Ningjia He1, Liang Zhang2* and Zhonghuai Xiang1*

Author Affiliations

1 The Key Sericultural Laboratory of Agricultural Ministry; the Key Laboratory for Sericultural Sciences and Genomics of the Ministry of Education, College of Biotechnology, Southwest University, Beibei, Chongqing 400715, China

2 National Engineering Center for Beijing Biochip Technology, Life Science Parkway, Changping District, Beijing 102206, China

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Genome Biology 2007, 8:R162  doi:10.1186/gb-2007-8-8-r162

Published: 4 August 2007

Abstract

We designed and constructed a genome-wide microarray with 22,987 70-mer oligonucleotides covering the presently known and predicted genes in the silkworm genome, and surveyed the gene expression in multiple silkworm tissues on day 3 of the fifth instar. Clusters of tissue-prevalent and tissue-specific genes and genes that are differentially expressed in different tissues were identified, and they reflect well major tissue-specific functions on the molecular level. The data presented in this study provide a new resource for annotating the silkworm genome.