Microarray-based gene expression profiles in multiple tissues of the domesticated silkworm, Bombyx mori

Qingyou Xia¹ , Daojun Cheng¹ , Jun Duan¹ , Genhong Wang¹ , Tingcai Cheng¹ , Xingfu Zha¹ , Chun Liu¹ , Ping Zhao¹ , Fangyin Dai¹ , Ze Zhang¹ , Ningjia He¹ , Liang Zhang² and Zhonghuai Xiang¹

¹The Key Sericultural Laboratory of Agricultural Ministry; the Key Laboratory for Sericultural Sciences and Genomics of the Ministry of Education, College of Biotechnology, Southwest University, Beibei, Chongqing 400715, China

²National Engineering Center for Beijing Biochip Technology, Life Science Parkway, Changping District, Beijing 102206, China

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Genome Biology 2007, 8:R162doi:10.1186/gb-2007-8-8-r162


Published:	4 August 2007

Subject areas: Development, Genome studies

Abstract

We designed and constructed a genome-wide microarray with 22,987 70-mer oligonucleotides covering the presently known and predicted genes in the silkworm genome, and surveyed the gene expression in multiple silkworm tissues on day 3 of the fifth instar. Clusters of tissue-prevalent and tissue-specific genes and genes that are differentially expressed in different tissues were identified, and they reflect well major tissue-specific functions on the molecular level. The data presented in this study provide a new resource for annotating the silkworm genome.