Method
Microarray-based gene expression profiles in multiple tissues of the domesticated silkworm, Bombyx mori
Author affiliations
1 The Key Sericultural Laboratory of Agricultural Ministry; the Key Laboratory for Sericultural Sciences and Genomics of the Ministry of Education, College of Biotechnology, Southwest University, Beibei, Chongqing 400715, China
2 National Engineering Center for Beijing Biochip Technology, Life Science Parkway, Changping District, Beijing 102206, China
Citation and License
Genome Biology 2007, 8:R162 doi:10.1186/gb-2007-8-8-r162
Published: 4 August 2007Abstract
We designed and constructed a genome-wide microarray with 22,987 70-mer oligonucleotides covering the presently known and predicted genes in the silkworm genome, and surveyed the gene expression in multiple silkworm tissues on day 3 of the fifth instar. Clusters of tissue-prevalent and tissue-specific genes and genes that are differentially expressed in different tissues were identified, and they reflect well major tissue-specific functions on the molecular level. The data presented in this study provide a new resource for annotating the silkworm genome.
