Table 1 |
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Primers used for determination of mRNA expression levels by Q-RT-PCR |
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|
Gene |
Forward primer |
Reverse primer |
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Mouse |
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|
Gapdh |
TGCACCACCAACTGCTTAG |
GATGCAGGGATGATGTTC |
|
Oct4 (Pou5f1) |
CCAATCAGCTTGGGCTAGAG |
CCTGGGAAAGGTGTCCTGTA |
|
Pecam |
GTCATGGCCATGGTCGAGTA |
CTCCTCGGCGATCTTGCTGAA |
|
Lyve-1 |
AGGAGCCCTCTCCTTACTGC |
ACCTGGAAGCCTGTCTCTGA |
|
vWF |
GCCAAAGATCTGGAACAGTGT |
GATGGAGAGGTTACACATCTC |
|
F2 |
CAGCTATGAGGAGGCCTTTG |
TCACACCCAGATCCATAGCA |
|
Tat |
TTAAGTCCAATGCGGACCTC |
GCTCTGTGAATTCCACGTCA |
|
Afp |
GCCCTACAGACCATGAAACAAG |
GTGAAACAGACTTCCTGGTCCT |
|
Ttr |
CTTTGCCTCTGGGAAGACC |
CAGAGTCGTTGGCTGTGAAA |
|
Foxa2 |
CCCGGGACTTAACTGTAACG |
TCATGTTGCTCACGGAAGAG |
|
Hnf4a |
GGTCAAGCTACGAGGACAGC |
ATGTACTTGGCCCACTCGAC |
|
Sox7 |
CTTCAGGGGACAAGAGTTCG |
CCATGACTTTCCCAGCATCT |
|
Sox17 |
CACAACGCAGAGCTAAGCAA |
TTGTAGTTGGGGTGGTCCTG |
|
Myocd |
CTGTGTGGAGTCCTCAGGTCAAACC |
GATGTGTTGCGGGCTCTTCAG |
|
Pdgra |
ACGTTCAAGACCAGCGAGTT |
CCTCCAGCATGGTGATACCT |
|
Pdgfrb |
CACCTTCTCCAGTGTGCTGA |
GGAGTCCATAGGGAGGAAGC |
|
Tek |
AAGCATGCCCATCTGGTTAC |
GCCTGCCTTCTTTCTCACAC |
|
Fbx15 |
GGCCTTGAATGGAGAACTGA |
TCAAACCACCCTAGGTCTGC |
|
Dnmt3l |
CCTGGTGAAGAACTGCCTTC |
GCAAAGTGAGCTGCACAGAG |
|
Rex1 |
CCTGCACACAGAAGAAAGCA |
TCAGTCTGTCGAGGGCTCTT |
|
Dppa5 |
CAGTCGCTGGTGCTGAAATA |
TCCATTTAGCCCGAATCTTG |
|
Eras |
CCCTGCTTGTCCATGAGATT |
TGGTAACTTGGTCGGAGAGG |
|
Utf1 |
GGCCATACCTTCGAATCCTC |
GGTTTGGTCGAAGGAACCTC |
|
Tdgf1 |
GCATCCTACGAGGGAGTTGA |
CACTGTGCTTGGCTGAAGAA |
|
Tcl1 |
TTCCTCTCTGGGTGTTCAGG |
ATCCCACACATTCCCTTTCA |
|
Ecat1 |
GAATGCCTGGAAGATCCAAA |
AAATCTCAGCTCGCCTTTCA |
|
Nanog |
GAGTGTGGGTCTTCCTGGTC |
GAGGCAGGTCTTCAGAGGAA |
|
Gdf3 |
CGCAGGACTTATGCTACGTG |
CTGGGCCATGGTCAACTT |
|
Fgf4 |
CCGACGAGTGTAAATTCAAAGAA |
GGAAGTGGGTTACCTTCATGG |
|
Sox2 |
ACCAGCTCGCAGACCTACAT |
AGTGGGAGGAAGAGGTAACCA |
|
Sox1 |
CACAACTCGGAGATCAGCAA |
TGTAATCCGGGTGTTCCTTC |
|
Pax6 |
TCAGACCTCCTCATACTCGTGCA |
TGTAGGTATCATAACTCCGCCCA |
|
Rat |
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|
Gapdh |
TGCACCACCAACTGCTTAG |
GATGCAGGGATGATGTTC |
|
Oct4 (Pou5f1) |
CTGTAACCGGCGCCAGAA |
TGCATGGGAGAGCCCAGA |
|
Vecad |
GGCCAACGAATTGGATTCTA |
GTTTACTGGCACCACGTCCT |
|
Flt-1 |
TGGCCAGAGGCATGGAGT |
TCGCAAATCTTCACCACATTG |
|
Flk-1 |
CCAAGCTCAGCACACAAAAA |
CCAACCACTCTGGGAACTGT |
|
vWF |
CCCACCGGATGGCTAGGTATT |
GAGGCGGATCTGTTTGAGGTT |
|
Afp |
ACCTGACAGGGAAGATGGTG |
GCAGTGGTTGATACCGGAGT |
|
Alb |
CTGGGAGTGTGCAGATATCAGAGT |
GAGAAGGTCACCAAGTGCTGTAGT |
|
Tat |
AACCTCAGCACCAATGTTCC |
TCTTCAGAGCACCCTGGACT |
|
Ttr |
CAGCAGTGGTGCTGTAGGAGTA |
GGGTAGAACTGGACACCAAATC |
|
Sox2 |
AACCCCAAGATGCACAACTC |
CCGGGAAGCGTGTACTTATC |
|
Otx1 |
GTTCGCAAAGACTCGCTACC |
CCGGAGACGACTTCTTCTTG |
|
Blbp |
CCAGCTGGGAGAAGAGTTTG |
TTTCTTTGCCATCCCACTTC |
|
Pax6 |
GTCCATCTTTGCTTGGGAAA |
TAGCCAGGTTGCGAAGAACT |
|
|
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|
Ulloa-Montoya et al. Genome Biology 2007 8:R163 doi:10.1186/gb-2007-8-8-r163 |
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