Table 1 |
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|
BAC library fingerprinting summary |
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|
Library |
Library indicator* |
Library construction enzyme† |
Source DNA |
Clones fingerprinted |
Successful fingerprints |
Clones in FPC‡ |
Average size (kb)§ |
Clone depth |
|
|
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|
CHORI240 |
E |
MboI |
Hereford bull L1 Domino 99373 |
200,064 |
170,644 |
169,283 |
169 |
9.5X |
|
RPCI42 |
H |
EcoRI/EcoRI methylase |
Holstein bull |
94,848 |
83,627 |
81,437 |
171 |
4.6X |
|
TAMBT |
T |
HindIII |
Angus bull, Angus cow |
44,928 |
40,380 |
40,077 |
106 |
1.4X |
|
|
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|
Total |
339,840 |
294,651 |
290,797 |
161 |
15.5X |
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|
|
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|
*The one-letter prefix used in clone names to indicate the library source. †All libraries were constructed from partially digested DNA. ‡After filtering. §Based on the sum of the sizes of the HindIII fingerprint fragments identified for each clone, excluding vector-specific fragments. |
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|
Snelling et al. Genome Biology 2007 8:R165 doi:10.1186/gb-2007-8-8-r165 |
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