Analysis of the relationship between shorter 3' UTRs and increased repression. The error bars for observed and expected data are based on the distribution of RE values and the distribution of the permutated data, respectively. (a) Shorter 3' UTRs in target genes are more strongly repressed by their predicted cognate miRNAs. (b) The expected RE values (computed using permutation testing) show minimal deviation from 1.0, representing a lack of repression. (c) This trend is increasingly exaggerated when subsets of miRNAs containing larger expression ratios between groups A and B are used, especially in 3' UTRs shorter than 200 bp. (d) The same trend of increased repression in shorter 3' UTRs is observed using a different target prediction algorithm, rna22.
Hon and Zhang Genome Biology 2007 8:R166 doi:10.1186/gb-2007-8-8-r166