Analysis of site and gene features that affect miRNA repression. The observed values are shown in black; the expected values (computed using permutation testing) are shown in gray. The error bars for observed and expected data are based on the distribution of RE values and the distribution of the permutated data, respectively. (a) Target genes with more binding sites are more strongly repressed. (b) Pairs of binding sites targeted by the same miRNA that are between 16 and 30 bp apart (by start positions) have significantly increased repression (asterisks shown for emphasis). (c) Genes that have multiple pairs of extensively overlapping sites, defined to be two binding sites responsive to the same miRNA whose start positions are within 10 bp of each other, have increased repression.
Hon and Zhang Genome Biology 2007 8:R166 doi:10.1186/gb-2007-8-8-r166