Research
The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells
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* Corresponding authors: Helle F Jørgensen helle.jorgensen@csc.mrc.ac.uk - Amanda G Fisher amanda.fisher@csc.mrc.ac.uk
1 Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, London W12 0NN, UK
2 Current address: Institute of Reproductive and Developmental Biology, Imperial College School of Medicine, London W12 0NN, UK
3 Developmental Epigenetics, MRC Clinical Sciences Centre, Imperial College School of Medicine, London W12 0NN, UK
4 Developmental Epigenetics, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XR, UK
Genome Biology 2007, 8:R169 doi:10.1186/gb-2007-8-8-r169
Published: 17 August 2007Additional files
Additional data file 1:
Supplementary Table 1 contains the percentages of cells positive for ES cell markers and supplementary Table 2 contains coordinates, GC content and line density of the genes analyzed in Figure 1b.
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Additional data file 2:
Supplementary Figure 1 shows alkaline phosphatase staining and cell cycle profiles of the mutant ES cell lines analyzed. Supplementary Figure 2 contains replication timing individual profiles of all genes in each of the mutant ES cell lines analyzed. Supplementary Figure 3 shows analysis of the Mage a2 gene. Supplementary Figure 4 shows analysis of short hairpin RNA mediated knockdown of Eed in Dnmt1 mutant ES cells.
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Additional data file 3:
Microarray data for the histone acetylation analysis.
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