The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells

Helle F Jørgensen¹ , Véronique Azuara¹^,2 , Shannon Amoils¹ , Mikhail Spivakov¹ , Anna Terry¹ , Tatyana Nesterova³ , Bradley S Cobb¹ , Bernard Ramsahoye⁴ , Matthias Merkenschlager¹ and Amanda G Fisher¹

¹Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, London W12 0NN, UK

³Developmental Epigenetics, MRC Clinical Sciences Centre, Imperial College School of Medicine, London W12 0NN, UK

⁴Developmental Epigenetics, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XR, UK

²Current address: Institute of Reproductive and Developmental Biology, Imperial College School of Medicine, London W12 0NN, UK

author email corresponding author email

Genome Biology 2007, 8:R169doi:10.1186/gb-2007-8-8-r169


Published:	17 August 2007

Subject areas: Cell biology, Molecular biology, Development, Genome studies

Additional files

Additional data file 1:

Supplementary Table 1 contains the percentages of cells positive for ES cell markers and supplementary Table 2 contains coordinates, GC content and line density of the genes analyzed in Figure 1b.

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Additional data file 2:

Supplementary Figure 1 shows alkaline phosphatase staining and cell cycle profiles of the mutant ES cell lines analyzed. Supplementary Figure 2 contains replication timing individual profiles of all genes in each of the mutant ES cell lines analyzed. Supplementary Figure 3 shows analysis of the Mage a2 gene. Supplementary Figure 4 shows analysis of short hairpin RNA mediated knockdown of Eed in Dnmt1 mutant ES cells.